2.1.1.157: sarcosine/dimethylglycine N-methyltransferase

This is an abbreviated version!
For detailed information about sarcosine/dimethylglycine N-methyltransferase, go to the full flat file.

Reaction

2 S-adenosyl-L-methionine +

Synonyms

dimethylglycine methyltransferase, DMT, DMT2, glycine sarcosine dimethylglycine methyltransferase, glycine sarcosine methyltransferase, GSDMT, GsSDMT, MXAN 3190, sarcosine dimethylglycine methyltransferas, sarcosine dimethylglycine methyltransferase, sarcosine dimethylglycine N-methyltransferase, Sdm, Sdm homologue protein, SdmA, SDMT

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.157 sarcosine/dimethylglycine N-methyltransferase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1	Activating Compound
256	AA Sequence
3	Application
1	CAS Registry Number
11	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
3	Expression
8	General Information
2	IC50 Value
23	Inhibitors
22	KM Value [mM]
3	Metals/Ions
12	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
18	Organism
4	Pathway
9	pH Optimum
1	pI Value
4	Protein Variants
6	Purification (Commentary)
6	Reaction
17	Reference
11	Specific Activity [micromol/min/mg]
1	Storage Stability
50	Substrates and Products (Substrate)
8	Subunits
34	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Inhibitors

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Inhibitors on EC 2.1.1.157 - sarcosine/dimethylglycine N-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Inhibitor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

4-chloromercuribenzoate

Halorhodospira halochloris

-

recombinant enzyme: 23% inhibition at 1.33 mM, completely reversible by 5.3 mM DTT

657578

betaine

3 entries

dimethylglycine

Aphanothece halophytica

Q83WC3

competitive inhibition, versus sarcosine, 40% inhibition at 250 mM

659286

isovaleric acid

Aphanothece halophytica

Q83WC3

10% inhibition at 250 mM, with dimethylglycine

659286

KCl/NaCl

Halorhodospira halochloris

-

recombinant enzyme: 32% inhibition at 0.33 M KCl and 0.66 M NaCl with sarcosine, and 25% inhibition at 0.33 M KCl and 0.66 M NaCl with dimethylglycine, decrease inhibition by betaine, overview

657578

L-alanine

Aphanothece halophytica

Q83WC3

10% inhibition at 250 mM, with dimethylglycine

659286

L-cysteine

Aphanothece halophytica

Q83WC3

10% inhibition at 250 mM, with dimethylglycine

659286

L-methionine

Aphanothece halophytica

Q83WC3

10% inhibition at 250 mM, with dimethylglycine

659286

L-phenylalanine

Aphanothece halophytica

Q83WC3

10% inhibition at 250 mM

659286

n-butyric acid

Aphanothece halophytica

Q83WC3

50% inhibition at 250 mM, with dimethylglycine

659286

propionic acid

Aphanothece halophytica

Q83WC3

10% inhibition at 250 mM, with dimethylglycine

659286

S-adenosyl-L-homocysteine

4 entries

sarcosine

Aphanothece halophytica

Q83WC3

slight competitive inhibition, versus dimethylglycine, 20% inhibition at 250 mM

659286

tert-butylacetic acid

Aphanothece halophytica

Q83WC3

30% inhibition at 250 mM, with dimethylglycine

659286

additional information

4 entries

-

betaine

Halorhodospira halochloris

-

recombinant enzyme: poor inhibition, 71% inhibition at 2 M with sarcosine, and 69% inhibition at 2 M with dimethylglycine, partially reversible by salts KCl and NaCl, overview

657578

betaine

Methanohalophilus portucalensis

-

above 1.0 M, no effect on sarcosine N-methyltransferase activity, 35% inhibition of N,N-dimethylglycine N-methyltransferase activity

695924

betaine

Methanohalophilus portucalensis

F6KV62

does not affect the SMT activity, but represses the DMT activity by 35% at 2 M

720836

S-adenosyl-L-homocysteine

Aphanothece halophytica

Q83WC3

product inhibition

659286

S-adenosyl-L-homocysteine

Halorhodospira halochloris

-

recombinant enzyme: competitive product inhibition, 50% inhibition at 0.5 mM with glycine, and at 2.3 mM with sarcosine

657578

S-adenosyl-L-homocysteine

Methanohalophilus portucalensis

-

0.2 mM, 63% loss of sarcosine N-methyltransferase activity, 81% loss of dimethylglycine N-methyltransferase activity. At 1 mM, complete loss of sarcosine N-methyltransferase activity, 88% loss of dimethylglycine N-methyltransferase activity

695924

S-adenosyl-L-homocysteine

Methanohalophilus portucalensis

F6KV62

product inhibition

720836

additional information

Aphanothece halophytica

Q83WC3

inhibition rates with sarcosine, overview, no inhibition with dimethylglycine by glycine, acetate, ethanolamine, monomethylethanolamine, serine, asparagine, glutamate, and proline

659286

-

additional information

Halorhodospira halochloris

-

no inhibition by N,N-dimethylglycine

657578

-

additional information

Methanohalophilus portucalensis

-

potassium or sodium ions at 200-1000 mM affect neither sarcosine N-methyltransferase activity nor dimethylglycine N-methyltransferase activity

695924

-

additional information

Methanohalophilus portucalensis

F6KV62

both the SMT and DMT activities of SDMT from halophilic methanoarchaea are not inhibited by KCl or NaCl

720836

-