2.1.1.156: glycine/sarcosine N-methyltransferase

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For detailed information about glycine/sarcosine N-methyltransferase, go to the full flat file.

Reaction

Synonyms

glycine sarcosine dimethylglycine methyltransferase, glycine sarcosine methyltransferase, glycine sarcosine N-methyltransferase, GSDMT, Gsm, GsmA, GSMT, GSMT2, MXAN 7068

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.156 glycine/sarcosine N-methyltransferase

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Results	in table
2	Activating Compound
702	AA Sequence
3	Application
1	CAS Registry Number
11	Cloned(Commentary)
2	Cofactor
4	Expression
10	General Information
1	General Stability
2	IC50 Value
21	Inhibitors
24	KM Value [mM]
5	Metals/Ions
17	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
24	Organism
4	Pathway
5	PDB ID
9	pH Optimum
2	pH Range
1	pI Value
16	Protein Variants
7	Purification (Commentary)
2	Reaction
16	Reference
10	Specific Activity [micromol/min/mg]
51	Substrates and Products (Substrate)
9	Subunits
28	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]

Purification

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Purification on EC 2.1.1.156 - glycine/sarcosine N-methyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Aphanothece halophytica

Q83WC4

659286

both native and recombinant protein

Methanohalophilus portucalensis

F6KV61

758143

by hydrophobic interaction chromatography, ammonium sulfate fractionation, gel filtration, ion exchange chromatography and ultrafiltration

Halorhodospira halochloris

Q9KJ22

659145

recombinant enzyme from Escherichia coli by ammonium sulfate fractionation and ion exchange chromatography to homogeneity

Aphanothece halophytica

Q83WC4

659286

recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, hydrophobic and anion exchange chromatography, and ultrafiltration to homogeneity

Halorhodospira halochloris

-

657578

recombinant MpGSMT from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Methanohalophilus portucalensis

F6KV61

720836

recombinant protein using His-tag

Parasynechococcus marenigrum WH 8102

Q7U4Z8

671647