2.1.1.156: glycine/sarcosine N-methyltransferase
This is an abbreviated version!
For detailed information about glycine/sarcosine N-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.156
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2.1.1.156
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halophilic
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betaine
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methanohalophilus
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portucalensis
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osmolyte
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s-adenosylhomocysteine
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feedback-inhibited
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methanogen
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methanoarchaea
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osmoprotectant
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methyltransferases
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phosphorylation-mediated
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biotechnology
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agriculture
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nutrition
- 2.1.1.156
-
halophilic
- betaine
-
methanohalophilus
- portucalensis
-
osmolyte
- s-adenosylhomocysteine
-
feedback-inhibited
-
methanogen
-
methanoarchaea
-
osmoprotectant
- methyltransferases
-
phosphorylation-mediated
- biotechnology
- agriculture
- nutrition
Reaction
Synonyms
glycine sarcosine dimethylglycine methyltransferase, glycine sarcosine methyltransferase, glycine sarcosine N-methyltransferase, GSDMT, Gsm, GsmA, GSMT, GSMT2, MXAN 7068
ECTree
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DNA and amino acid sequence determination, functional coexpression in Escherichia coli with sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, conferring betaine accumulation and increased salt tolerance to the Escherichia coli cells
DNA and amino acid sequence determination, the single gene encodes the glycine sarcosine methyltransferase as well as the sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, the latter being probably a proteolytic processing product of the first, poor expression in Escherichia coli of the full length and the truncated enzyme
expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS
Parasynechococcus marenigrum WH 8102
functional overexpression in Escherichia coli strain XL-1 Blue conferring betaine accumulation and increased salt tolerance when coexpressed with the sarcosine dimethylglycine methyltransferase, EC 2.1.1.157
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gene gsmA, DNA and amino acid sequence determination and analysis, sequence comparison
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gene Mpgsmt, DNA and amino acid sequence determination and analysis and sequence comparisons, phylogenetic analysis of GSMT, expression of complete gene cluster of Mpgsmt-sdmt in Escherichia coli strain BL21(DE3) and in strain MKH13 deficient in betaine transport, the recombinant enzyme is functional to synthesize and accumulate betaine and confers elevated survival ability in betaine transport deficient mutant strain MKH13 under high salt stress. The recombinantly expressed enzyme shows 10000fold lower catalytic efficiency compared with the enzyme isolated from the original cells
orf1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of wild-type and mutant enzymes in Escherichia coli, functional coexpression of the soluble enzyme with the sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, increases the intracellular betaine level and the growth rate of Escherichia coli strain BL21(DE3)