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2.1.1.151: cobalt-factor II C20-methyltransferase

This is an abbreviated version!
For detailed information about cobalt-factor II C20-methyltransferase, go to the full flat file.

Reaction

S-adenosyl-L-methionine
+
cobalt-factor II
=
S-adenosyl-L-homocysteine
+
cobalt-factor III

Synonyms

20-methyl transferase of corrin biosynthesis, CbiL, cbiL gene product, M-2, precorrin-2 methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.151 cobalt-factor II C20-methyltransferase

Crystallization

Crystallization on EC 2.1.1.151 - cobalt-factor II C20-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structures of Chlorobium tepidum CbiL and CbiL in complex with S-adenosylhomocysteine (the S-demethyl form of S-adenosylmethionine). CbiL forms a dimer in the crystal, and each subunit consists of N-terminal and C-terminal domains. S-Adenosylhomocysteine binds to a cleft between the two domains, where it is specifically recognized by extensive hydrogen bonding and van der Waals interactions
hanging-drop vapor-diffusion method, complex with S-adenosylhomocystein obtained by soaking of ready crystals in S-adenosylhomocystein solution
crystallized by hanging drop vapor diffusion method in the presence of S-adenosyl-L-methionine or S-adenosyl-L-homocysteine, crystals complemented with substrate by soaking in substrate solution, no crystal growth in the absence of S-adenosyl-L-methionine or S-adenosyl-L-homocysteine