2.1.1.151: cobalt-factor II C20-methyltransferase
This is an abbreviated version!
For detailed information about cobalt-factor II C20-methyltransferase, go to the full flat file.
Reaction
Synonyms
20-methyl transferase of corrin biosynthesis, CbiL, cbiL gene product, M-2, precorrin-2 methyltransferase
ECTree
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Crystallization
Crystallization on EC 2.1.1.151 - cobalt-factor II C20-methyltransferase
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crystal structures of Chlorobium tepidum CbiL and CbiL in complex with S-adenosylhomocysteine (the S-demethyl form of S-adenosylmethionine). CbiL forms a dimer in the crystal, and each subunit consists of N-terminal and C-terminal domains. S-Adenosylhomocysteine binds to a cleft between the two domains, where it is specifically recognized by extensive hydrogen bonding and van der Waals interactions
hanging-drop vapor-diffusion method, complex with S-adenosylhomocystein obtained by soaking of ready crystals in S-adenosylhomocystein solution
crystallized by hanging drop vapor diffusion method in the presence of S-adenosyl-L-methionine or S-adenosyl-L-homocysteine, crystals complemented with substrate by soaking in substrate solution, no crystal growth in the absence of S-adenosyl-L-methionine or S-adenosyl-L-homocysteine