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2.1.1.148: thymidylate synthase (FAD)

This is an abbreviated version!
For detailed information about thymidylate synthase (FAD), go to the full flat file.

Word Map on EC 2.1.1.148

Reaction

5,10-methylenetetrahydrofolate
+
dUMP
+
NADPH
+
H+
=
dTMP
+
tetrahydrofolate
+
NADP+

Synonyms

A674R, complementing thymidylate synthase, FDTS, flavin dependent thymidylate synthase, flavin-dependent thymidylate synthase, flavin-dependent thymidylate synthase X, flavin-dependent TS, Thy1, thymidylate synthase 1, thymidylate synthase complementing protein, thymidylate synthase ThyX, thymidylate synthase X, ThyX, thyX-encoded thymidylate synthase, TSCP, TTHA1096

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.148 thymidylate synthase (FAD)

Purification

Purification on EC 2.1.1.148 - thymidylate synthase (FAD)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by nickel chromatography and gel filtration, more than 95% pure
Paramecium bursaria Chlorella virus-1
-
from transformed E. coli
-
from transformed E. coli using His-tag
nickel affinity and ion-exchange chromatography
-
nickel chelating column chromatography, GSH-Sepharose column chromatography, and Superdex 200 gel filtration
on Ni-NTA column
-
to near homogeneity, by heat treatment at 85°C for 15 min, followed by Ni2+ column chromatography