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2.1.1.142: cycloartenol 24-C-methyltransferase

This is an abbreviated version!
For detailed information about cycloartenol 24-C-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.142

Reaction

S-adenosyl-L-methionine
+
5alpha-cholesta-8,24-dien-3beta-ol
=
S-adenosyl-L-homocysteine
+
24-methylene-5alpha-cholest-8-en-3beta-ol

Synonyms

(S)-adenosyl-L-methionine-DELTA24-sterol methyltransferase, (S)-adenosyl-L-methionine:DELTA24(25)-sterol methyltransferase, 24-SMT, C-24-methyltransferase, C24-sterol methyltransferase type 1, DELTA24(25)-sterol methyltransferase, DELTA24-sterol methyltransferase, Erg6, GhSMT2-1, GhSMT2-2, More, SAM-DELTA24 sterol transmethylase, SAM:SMT, SMT, SMT protein, SMT-2, SMT-3, SMT1, SMT2, SMT2-1, sterol 24-C methyltransferase, sterol 24C-methyltransferases, sterol C-24 methyltransferase, sterol C-24-methyltransferase, sterol C-methyltransferase, sterol C24-methyltransferase, sterol methyl transferase, sterol methyltransferase, sterol methyltransferase2, zymosterol-24(25)-methyltransferase, zymosterol-24-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.142 cycloartenol 24-C-methyltransferase

Engineering

Engineering on EC 2.1.1.142 - cycloartenol 24-C-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F82I
-
continues to catalyze strongly both the first and second C1-transfer activities and generates product sets similar to the control
F82L
-
first C1-transfer activity is relatively unaffected, loss of the second C1-transfer activity
F85L
-
first C1-transfer activity is relatively unaffected, loss of the second C1-transfer activity
F91L
-
first C1-transfer activity reduced from the control activity, loss of the second C1-transfer activity
F93L
-
first C1-transfer activity reduced from the control activity, loss of the second C1-transfer activity
W87L
-
first and second C1-transfer activities are greatly reduced from the control activities
Y81F
-
site-directed mutagenesis, the mutation causes a preferential change in affinity for DELTA24-sterols that affords alter partitioning in the direction of 24-ethyl(idene) product formation
Y81L
-
site-directed mutagenesis, the mutation causes a preferential change in affinity for DELTA24-sterols that affords alter partitioning in the direction of 24-ethyl(idene) product formation
Y88F
-
site-directed mutagenesis, the mutation causes a 10% loss in activity compared to the wild-type enzyme
Y88L
-
site-directed mutagenesis, the mutation causes a 50% loss in activity compared to the wild-type enzyme
Y81F
-
site-directed mutagenesis, the mutation causes a preferential change in affinity for DELTA24-sterols that affords alter partitioning in the direction of 24-ethyl(idene) product formation
-
Y81L
-
site-directed mutagenesis, the mutation causes a preferential change in affinity for DELTA24-sterols that affords alter partitioning in the direction of 24-ethyl(idene) product formation
-
Y88F
-
site-directed mutagenesis, the mutation causes a 10% loss in activity compared to the wild-type enzyme
-
Y88L
-
site-directed mutagenesis, the mutation causes a 50% loss in activity compared to the wild-type enzyme
-
additional information