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2.1.1.141: jasmonate O-methyltransferase

This is an abbreviated version!
For detailed information about jasmonate O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.141

Reaction

S-adenosyl-L-methionine
+
jasmonate
=
S-adenosyl-L-homocysteine
 +
methyl jasmonate

Synonyms

AtJMT, CaJMT, carboxyl methyltransferase, JA carboxyl methyltransferase, JA methyltransferase, JAMT, jasmonic acid carboxyl methyltransferase, jasmonic acid methyltransferase, JMT, JMT protein, JMT1, PaJAMT1, PaJAMT2, PaJAMT3, PaSABATH10, PaSABATH4, PaSABATH5, S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase, SABATH3

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.141 jasmonate O-methyltransferase

General Information

General Information on EC 2.1.1.141 - jasmonate O-methyltransferase

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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
the enzyme belongs to the protein family of SABATH methyltransferases, ten genes encode isozymes PaSABATH1-10. Five of the PaSABATH isozymes (PaSABATH3, PaSABATH6, PaSABATH7, PaSABATH8, and PaSABATH9) do not show activity with any of the four substrates, i.e. indole-3-acetic acid, jasmonic acid, giberellic acid A3, and salicylic acid, the other five of the PaSABATHs each show activity with one or more of the four substrates. PaSABATH1 has the highest level of specific activity with indole-3-acetic acid and is renamed as PaIAMT (EC 2.1.1.278). PaSABATH2 has the highest level of specific activity with salicylic acid and is designated as PaSAMT (EC 2.1.1.274). For comparison, PaSAMT is also assayed with two compounds of similar structure benzoic acid and anthranilic acid (cf. EC 2.1.1.273). While PaSAMT has no activity with anthranilic acid, its activity with benzoic acid is approximately 8% of that with salicylic acid. PaSABATH4, PaSABATH5 and PaSABATH10 show the highest level of specific activity with jasmonic acid and are renamed PaJAMT1, PaJAMT2, and PaJAMT3, respectively (EC 2.1.1.141). Their products are confirmed to be methyljasmonate
metabolism
overexpression of enzyme in the Glycine max seeds results in decreased amounts of tryptophan, palmitic acid, linolenic acid, and stachyose, but increased levels of gadoleic acid and genistein. In particular, seeds contain 120.0-130.5% more genistein and 60.5-82.1% less stachyose than the wild type
physiological function
additional information
-
structural modeling of isozymes PaJAMT1 and PaJAMT3, docking structures with Indole-3-acetic acid (IAA) suggest that the active-site residues (e.g. V339 and F343 from PaJAMT1 and M347 and F351 from PaJAMT3) interfere with IAA binding for the methyl transfer, and this is consistent with the relatively low activities of these two enzymes toward IAA.When jasmonate (JA) is docked into the active sites of PaJAMT1 and PaJAMT3, the steric clashes with the active site residues are not observed, and this observation structurally validates that they are jasmonate methyltransferases, JAMTs