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2.1.1.137: arsenite methyltransferase

This is an abbreviated version!
For detailed information about arsenite methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.137

Reaction

2 S-adenosyl-L-methionine +

arsenic triglutathione
+ 2 thioredoxin +
H2O
=
S-adenosyl-L-homocysteine
 +
dimethylarsinous acid
 + 3 glutathione + 2 thioredoxin disulfide

Synonyms

AdoMet:arsenic(III) methyltransferase, arsenic (+3 oxidation state) methyltransferase, arsenic (+3 oxidation state)-methyltransferase, arsenic (+3) methyltransferase, arsenic (III) methyltransferase, arsenic (III) S-adenosylmethionine methyltransferase, arsenic methyltransferase Cyt19, arsenic(III) methyltransferase, arsenite (+3 oxidation state) methyltransferase, arsenite methyltransferase, arsenite S-adenosylmethionine methyltransferase, ArsM, ArsM protein, ArsM7B, As(III) methyltransferase, As(III) S-adenosylmethionine methyltransferase, As(III) SAM methyltransferase, AS3MT, AsIII S-adenosylmethionine methyltransferase, Cyr19, Cyt19, EC 2.1.1.138, hAS3MT, methylarsonite methyltransferase, N6AMT1, S-adenosyl-L-methionine:arsenic(III) methyltransferase, S-adenosyl-L-methionine:methylarsonite As-methyltransferase, S-adenosylmethionine dependent arsenic methyltransferase, [As(III)] methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.137 arsenite methyltransferase

Purification

Purification on EC 2.1.1.137 - arsenite methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE anion exchange chromatography, Sephadex G-200, High Q anion exchange chromatographie, 40fold purification
-
DEAE cellulose, ammonium sulfate precipitation, Sephadex G-200, Sephadex G-100, 2000fold purification
-
DEAE-cellulose, Sephadex G-200, Sephadex G-100, anion exchange chromatographie
-
HisTrap column chromatography
-
native enzyme several thousandfold using pH-dependent fractionation, chromatofocusing, and S-adenosylhomocysteine-affinity chromatography, the purification of AS3MT from liver cytosol is dependent on the presence of 1 mM dithiothreitol and 5 mM GSH
Ni Sepharose column chromatography, gel filtration
-
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
nickel affinity His GraviTrap column chromatography
recombinant enzyme
-