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2.1.1.137: arsenite methyltransferase

This is an abbreviated version!
For detailed information about arsenite methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.137

Reaction

2 S-adenosyl-L-methionine +

arsenic triglutathione
+ 2 thioredoxin +
H2O
=
S-adenosyl-L-homocysteine
 +
dimethylarsinous acid
 + 3 glutathione + 2 thioredoxin disulfide

Synonyms

AdoMet:arsenic(III) methyltransferase, arsenic (+3 oxidation state) methyltransferase, arsenic (+3 oxidation state)-methyltransferase, arsenic (+3) methyltransferase, arsenic (III) methyltransferase, arsenic (III) S-adenosylmethionine methyltransferase, arsenic methyltransferase Cyt19, arsenic(III) methyltransferase, arsenite (+3 oxidation state) methyltransferase, arsenite methyltransferase, arsenite S-adenosylmethionine methyltransferase, ArsM, ArsM protein, ArsM7B, As(III) methyltransferase, As(III) S-adenosylmethionine methyltransferase, As(III) SAM methyltransferase, AS3MT, AsIII S-adenosylmethionine methyltransferase, Cyr19, Cyt19, EC 2.1.1.138, hAS3MT, methylarsonite methyltransferase, N6AMT1, S-adenosyl-L-methionine:arsenic(III) methyltransferase, S-adenosyl-L-methionine:methylarsonite As-methyltransferase, S-adenosylmethionine dependent arsenic methyltransferase, [As(III)] methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.137 arsenite methyltransferase

Cloned

Cloned on EC 2.1.1.137 - arsenite methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
after expression in COS-1 cells and correction for transfection efficiency, the Trp173 allozyme displays 31%, Thr287 350%, Ile306 4.8%, and Thr287/Ile306 6.2% of the activity of the wild type allozyme, with 20, 190, 4.4, and 7.9% of the level of wild-type immunoreactive protein, respectively
-
codon-optimized expression in Bacillus subtilis
creation of a clonal human UROtsa cell line (UROtsa/F35) that expresses rat AS3MT and, unlike the parent UROtsa cell line, methylates inorganic arsenite and methylarsenite
-
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, genotyping with method development and optimization, overview
expressed in an arsenic-sensitive strain of Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) pLysS cells
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli DH10B cells
expressed in Escherichia coli DH5alpha and AW3110 cells
-
expressed in the arsenite-sensitive Escherichia coli strain AW3110(DE3)
expression in UROtsa cell
expression of a mutant lacking 31 residues from the N-terminus and 28 residues from the C-terminus
expression of rat AS3MT in a simian virus 40 (SV40)-transformed human urothelial cell line confers the capacity to methylate inorganic arsenic on cells that otherwise do not express AS3MT and that have a null phenotype for iAs methylation
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT
gene arsM, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotype-phenotype correlations for arsenic methylation and AS3MT, recombinant expression
genes arsM and arsMC2, functional expression in enzyme-deficient Escherichia coli strain AW3110
-
genotyping
wild-type ArsM and the C281/282S ArsMC2 variant, both with a His6 tag, are expressed in Escherichia coli
-