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2.1.1.128: (RS)-norcoclaurine 6-O-methyltransferase

This is an abbreviated version!
For detailed information about (RS)-norcoclaurine 6-O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.128

Reaction

S-adenosyl-L-methionine
+
(RS)-norcoclaurine
=
S-adenosyl-L-homocysteine
 +
(RS)-coclaurine

Synonyms

(R,S)-norcoclaurine 6-O-methyltransferase, (S)-Coclaurine N-methyltransferase, (S)-norcoclaurine 6-O-methyltransferase, (S)-norcoclaurine-6-O-methyltransferase, 3’-hydroxy-N-methylcoclaurine 4’-O-methyltransferase, 6-OMT, 6OMT, 6OMT1, 6OMT2, 7OMT, Ec4’OMT, Methyltransferase, (S)-coclaurine N-, Methyltransferase, norlaudanosoline, NLS-OMT, Norcoclaurine 6-O-methyltransferase, Ps6OMT, S-Adenosyl-L-methionine:(S)-coclaurine-N-methyltransferase, S-Adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase, S-Adenosylmethionine:(R),(S)-norlaudanosoline-6-O-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.128 (RS)-norcoclaurine 6-O-methyltransferase

Cloned

Cloned on EC 2.1.1.128 - (RS)-norcoclaurine 6-O-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid
expressed as His-tag fusion protein in Escherichia coli ER2566pLys S
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Eschscholzia californica
expression in Escherichia coli
full-length cDNA of Coptis japonica 6OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens
into the pET-21d vector for expression in Escherichia coli BL21DE3 cells