2.1.1.117: (S)-scoulerine 9-O-methyltransferase

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For detailed information about (S)-scoulerine 9-O-methyltransferase, go to the full flat file.

Reaction

Synonyms

(S)-Adenosyl-L-methionine:(S)-scoularine 9-O-methyltransferase, (S)-scoulerine 9-O-methyltransferase, Methyltransferase, (S)-scoularine 9-, Methyltransferase, (S)-scoularine 9- (Coptis japonica clone pCJSMT), scoulerine 9-O-methyltransferase, scoulerine 9-OMT, scoulerine-9-O-methyltransferase, scoulerine/reticuline O-methyltransferase, SMT, SOMT, SOMT1

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.117 (S)-scoulerine 9-O-methyltransferase

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Results	in table
18	AA Sequence
2	Application
2	CAS Registry Number
4	Cloned(Commentary)
1	General Information
16	Inhibitors
10	kcat/KM [mM/s]
15	KM Value [mM]
1	Localization
1	Metals/Ions
4	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
14	Organism
7	Pathway
8	PDB ID
3	pH Optimum
2	pH Range
1	pI Value
2	Purification (Commentary)
1	Reaction
1	Reaction Type
14	Reference
9	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
28	Substrates and Products (Substrate)
3	Subunits
15	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
10	Turnover Number [1/s]

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Application on EC 2.1.1.117 - (S)-scoulerine 9-O-methyltransferase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

analysis

Coptis japonica

Q39522

transient RNA silencing of scoulerine 9-O-methyltransferase expression by double stranded RNA in Coptis japonica protoplasts. Utility of gene silencing based on in vitro synthesized dsRNA to study function and behavior of endogenous enzymes

658313

medicine

Thalictrum flavum subsp. glaucum

Q5C9L2

a Saccharomyces cerevisiae strain is engineered to express seven heterologous enzymes (Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR)), resulting in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor. A number of strategies are implemented to improve flux through the pathway, including enzyme variant screening, genetic copy number variation, and culture optimization. This leads to an over 70-fold increase in canadine titer up to 1.8 mg/l. Increased canadine titers enable extension of the pathway to produce berberine, a major constituent of several traditional medicines in a microbial host. This strain is viable at pilot scale

743234