2.1.1.116: 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase

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For detailed information about 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase, go to the full flat file.

Reaction

Synonyms

(R,S)-3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase, (S)-3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase, (S)-3'-hydroxy-N-methylcoclaurine 4-O-methyltransferase, (S)-3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase, 3'-hydroxy N-methylcoclaurine 4'-O-methyltransferase, 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase, 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase, 3-hydroxy-N-methylcoclaurine 4-O-methyltransferase, 4'-OMT, 4'OMT, HOMT, Methyltransferase, (S)-3'-hydroxy-N-methylcoclaurine 4'-, Ps4'OMT, S-Adenosyl-L-methionine: 3'-hydroxy-N-methyl-(S)-coclaurine-4'-O-methyl transferase, S-adenosyl-L-methionine:(R,S)-3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase, S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase, SAM:3'-hydroxy-N-methyl-(S)-coclaurine-4'-O-methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.116 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase

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Results	in table
6	Activating Compound
10	AA Sequence
3	Application
1	CAS Registry Number
7	Cloned(Commentary)
2	General Information
7	Inhibitors
8	KM Value [mM]
2	Localization
6	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
16	Organism
6	Pathway
5	pH Optimum
1	pH Range
5	Purification (Commentary)
1	Reaction
7	Reaction Type
16	Reference
16	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
21	Substrates and Products (Substrate)
2	Subunits
29	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
2	Temperature Stability [°C]

Cloned

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Cloned on EC 2.1.1.116 - 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid

Papaver somniferum

Q7XB10

743234

full-length cDNA of Coptis japonica 4'OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens

Coptis japonica

Q9LEL5

676471

into pRSETB and introduced into Escherichia coli strain ER2566 pLys S

Papaver somniferum

-

670561

into the pET-21d vector for expression in Escherichia coli BL21DE3 cells

Coptis japonica

Q9LEL5

676471

into the pET-41a+ vector for expression in Escherichia coli BL21DE3 cells

Eschscholzia californica

-

676471

into vector pQE31 containing six histidines as an N-terminal tag and overexpressed in Escherichia coli SG13009

Papaver somniferum

-

676691

isolation of molecular clone

Papaver somniferum

Q7XB10, Q7XB11

660096