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2.1.1.11: magnesium protoporphyrin IX methyltransferase

This is an abbreviated version!
For detailed information about magnesium protoporphyrin IX methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.11

Reaction

S-adenosyl-L-methionine
+
magnesium protoporphyrin IX
=
S-adenosyl-L-homocysteine
+
magnesium protoporphyrin IX 13-methyl ester

Synonyms

(-)-S-adenosyl-L-methionine:magnesium-protoporphyrin IX methyltransferase, BchM, ChlM, LOC_Os06g04150, magnesium protoporphyrin IX methyltransferase, magnesium protoporphyrin methyltransferase, magnesium protoporphyrin O-methyltransferase, methyltransferase, magnesium protoporphyrin, Mg-protoporphyrin IX methyltransferase, MgPIXMT protein, MgPMT, S-adenosyl-L-methionine:Mg protoporphyrin methyltransferase, Ygl18

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.11 magnesium protoporphyrin IX methyltransferase

Engineering

Engineering on EC 2.1.1.11 - magnesium protoporphyrin IX methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C111S
0.1% of wild-type activity
C111S/C115S
only residual in vitro activity under reducing conditions, almost complete loss of activity under non-reducing conditions
C115S
20% of wild-type activity, strong stimulation by dithiothreitol
C115S/C177S
only residual in vitro activity under reducing conditions, almost complete loss of activity under non-reducing conditions
C177S
residue is responsible for redox-dependent enzyme activation. In absence of dithiothreitol, increased activity compared with wild-type. Only slight activation by dithiothreitol
L271P
-
naturally occuring mutation, Mg-protoporphyrin IX accumulated in the mutant, a low activity of the mutant protein is not excluded
L225F
-
mutation yellow-green leaf 18, leads to yellow-green leaves, decreased chlorophyll level, and climate-dependent growth differences
H139A
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
H139N
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
H139Q
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Y28A
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein
Y28F
mutation leads to inclusion body formation during bacterial expression