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2.1.1.104: caffeoyl-CoA O-methyltransferase

This is an abbreviated version!
For detailed information about caffeoyl-CoA O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.104

Reaction

S-adenosyl-L-methionine
+
caffeoyl-CoA
=
S-adenosyl-L-homocysteine
 +
feruloyl-CoA

Synonyms

ASCCoAOMT, At4g26220, caffeic acid 3-O-methyltransferase, caffeoyl CoA 3-O-methyltransferase, caffeoyl CoA methyltransferase, caffeoyl CoA methyltransferase 7, caffeoyl CoA O-methyltransferase, caffeoyl CoA-O-methyltransferase, caffeoyl coenzyme A 3-O-methyltransferase, caffeoyl coenzyme A methyltransferase, caffeoyl coenzyme A O-methyltransferase, caffeoyl-CoA 3-O-methyltransferase, caffeoyl-CoA-O-methyl transferase 1, caffeoyl-CoA-O-methyl transferase 2, caffeoyl-coenzyme A 3-o-methyltransferase, caffeoyl-coenzyme A O-methyltransferase, CCoAMT, CCoAOMT, CCoAOMT 1, CCoAOMT 2, CCoAOMT-1, CCoAOMT-like, CCoAOMT1, CCoAOMT2, CCoAOMT7, CCOMT, COMT, F6OMT, hi12, More, O-methyltransferase, OMT-15, OMT-17, OMT1, S-adenosyl-L-methionine:trans-caffeoyl-CoA 3-O-methyltransferase, TaCCoAOMT1, trans-caffeoyl-CoA 3-O-methyltransferase, Vp-OMT4, Vp-OMT5

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.104 caffeoyl-CoA O-methyltransferase

Purification

Purification on EC 2.1.1.104 - caffeoyl-CoA O-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on Blue Sepharose CL-6B, isoelectric focusing
-
by nickel affinity chromatography
by sonication, centrifugation and Ni2+-NTA column, more than 98% pure
glutathione Sepharose 4 column chromatography
-
glutathione Sepharose column chromatography
mutant and wild-type protein
Ni-NTA column chromatography, Resource-Q column chromatography and hydroxyapatite column chromatography
Ni-NTA Sefinose column chromatography
nickel column chromatography
partially purified
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, expression of antisense enzyme in transgenic Nicotiana tabacum plants
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3), removal of the His-tag
using Ni-NTA chromatography