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2.1.1.104: caffeoyl-CoA O-methyltransferase

This is an abbreviated version!
For detailed information about caffeoyl-CoA O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.104

Reaction

S-adenosyl-L-methionine
+
caffeoyl-CoA
=
S-adenosyl-L-homocysteine
 +
feruloyl-CoA

Synonyms

ASCCoAOMT, At4g26220, caffeic acid 3-O-methyltransferase, caffeoyl CoA 3-O-methyltransferase, caffeoyl CoA methyltransferase, caffeoyl CoA methyltransferase 7, caffeoyl CoA O-methyltransferase, caffeoyl CoA-O-methyltransferase, caffeoyl coenzyme A 3-O-methyltransferase, caffeoyl coenzyme A methyltransferase, caffeoyl coenzyme A O-methyltransferase, caffeoyl-CoA 3-O-methyltransferase, caffeoyl-CoA-O-methyl transferase 1, caffeoyl-CoA-O-methyl transferase 2, caffeoyl-coenzyme A 3-o-methyltransferase, caffeoyl-coenzyme A O-methyltransferase, CCoAMT, CCoAOMT, CCoAOMT 1, CCoAOMT 2, CCoAOMT-1, CCoAOMT-like, CCoAOMT1, CCoAOMT2, CCoAOMT7, CCOMT, COMT, F6OMT, hi12, More, O-methyltransferase, OMT-15, OMT-17, OMT1, S-adenosyl-L-methionine:trans-caffeoyl-CoA 3-O-methyltransferase, TaCCoAOMT1, trans-caffeoyl-CoA 3-O-methyltransferase, Vp-OMT4, Vp-OMT5

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.104 caffeoyl-CoA O-methyltransferase

Engineering

Engineering on EC 2.1.1.104 - caffeoyl-CoA O-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G46Y
mutation is sufficient for a reversal of the unusual para- back to meta-O-methylation of flavanones and dihydroflavonols
G46Y/W193F/R194M/I195K
methylation in meta-position is preferred, mutations reduce the affinity for eriodictyol due to almost no interactions of the side chains with the substrate
D228A
only residual enzyme activities are detectable
K157A
mutation decreases Km but not the kcat value of eriodictyol, a single, unusual product is formed, namely the yellow fluorescing quercetagetin 5-O-methyl ether
N181A
complete loss of activity with eriodictyol
D58A
-
decrease of catalytic activity
Q61S
-
decrease of catalytic activity
R220T
-
total loss of activity, R220 is involved in the electrostatic interaction with CoA-moiety of the substrate
D168L
-
the mutation abolishes the enzyme activity of OMT-15
D194L
-
the mutation abolishes the enzyme activity of OMT-15
D209L
-
the mutation abolishes the enzyme activity of OMT-17
D234L
-
the mutation abolishes the enzyme activity of OMT-17
E69L
-
the mutation results in about 14% loss of enzyme activity in OMT-15 and 40% loss of activity in OMT-17
N195I
-
the mutation abolishes the enzyme activity of OMT-15
N235I
-
the mutation abolishes the enzyme activity of OMT-17
L110A
the mutant shows about 9% of wild type activity with caffeic acid as substrate
L110G
the mutant shows about 9% of wild type activity with caffeic acid as substrate
L110S
the mutant shows about 9% of wild type activity with caffeic acid as substrate
L110V
the mutant shows about 29% of wild type activity with caffeic acid as substrate
L110Y
the mutant shows about 77% of wild type activity with caffeic acid as substrate
T51A
the mutation confers resistance to the bacterium Ralstonia solanacearum, resistance in F2 population is derived from a cross between a bacterial wilt-resistant variety, T51A, and a bacterial wilt-susceptible variety, T9230
D252A
inactive
K180A
inactive
additional information