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2.1.1.103: phosphoethanolamine N-methyltransferase

This is an abbreviated version!
For detailed information about phosphoethanolamine N-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.103

Reaction

S-adenosyl-L-methionine
+
Ethanolamine phosphate
=
S-adenosyl-L-homocysteine
+
N-methylethanolamine phosphate

Synonyms

AtNMT1, DPR2, More, NMT1, NMT2, PEAMT, PEAMT1, Pfpmt, phosphodimethylethanolamine methyltransferase, phosphoethanolamine methyltransferase, phosphoethanolamine N-methyltransferase, phosphoethanolamine-N-methyltransferases, phosphomethylethanolamine N-methyltransferase, PMEAMT, PMT, PMT-1, PMT1, PMT2, PVX_083045, S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase, SoPEAMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.103 phosphoethanolamine N-methyltransferase

Purification

Purification on EC 2.1.1.103 - phosphoethanolamine N-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from E. coli, His-tag used for affinity chromatography
HisTrap column chromatography
Ni-NTA agarose column chromatography and Superdex 75 gel filtration
-
Ni-NTA column chromatography, gel filtration
-
Ni2+-nitrilotriacetic acid-agarose resin column chromatography and Superdex-200 gel filtration
-
nickel affinity column chromatography
nickel HisTrap column chromatography and Superdex 75PG gel filtration
nickel-affinity column chromatography and gel filtration
purification of the recombinant protein by affinity chromatography is found to be problematic and subject to losses in enzyme activity. The inclusion of DTT, BSA, and EDTA prevents the loss of enzyme activity during elution from a nickel ion matrix
recombinant His6-tagged PMT from Escherichia coli strain Rosetta II (DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin, followed by benzamidine adsorption chromatography, and gel filtration
-
recombinant wild-type and mutant enzymes from Escherichia coli by affinity chromatography