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2.1.1.103: phosphoethanolamine N-methyltransferase

This is an abbreviated version!
For detailed information about phosphoethanolamine N-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.103

Reaction

S-adenosyl-L-methionine
+
Ethanolamine phosphate
=
S-adenosyl-L-homocysteine
+
N-methylethanolamine phosphate

Synonyms

AtNMT1, DPR2, More, NMT1, NMT2, PEAMT, PEAMT1, Pfpmt, phosphodimethylethanolamine methyltransferase, phosphoethanolamine methyltransferase, phosphoethanolamine N-methyltransferase, phosphoethanolamine-N-methyltransferases, phosphomethylethanolamine N-methyltransferase, PMEAMT, PMT, PMT-1, PMT1, PMT2, PVX_083045, S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase, SoPEAMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.103 phosphoethanolamine N-methyltransferase

Engineering

Engineering on EC 2.1.1.103 - phosphoethanolamine N-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E8A
mutation of NMT2 isoform 2, loss of activity
K13A
mutation of NMT2 isoform 2, loss of activity
R9A
mutation of NMT2 isoform 2, loss of activity
W16A
mutation of NMT2 isoform 2, loss of activity
Y15A
mutation of NMT2 isoform 2, loss of activity
D110A
the mutant shows about 37% of wild type activity
D110N
the mutant shows about 80% of wild type activity
D128A
D128E
D128N
D128Q
compared to the wild type enzyme, the mutant shows almost complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 89% activity)
D61E
site-directed mutagenesis, inactive mutant, which fails to complement the mutant Saccharomyces cerevisiae strain pem1DELTApem2DELTA, overview
G153A
site-directed mutagenesis, the PfPMT mutant complements the mutant Saccharomyces cerevisiae strain pem1DELTApem2DELTA, overview
G63A
site-directed mutagenesis, the PfPMT mutant complements the mutant Saccharomyces cerevisiae strain pem1DELTApem2DELTA, overview
G83A
site-directed mutagenesis, inactive mutant, which fails to complement the mutant Saccharomyces cerevisiae strain pem1DELTApem2DELTA, overview
I86A
the mutant shows about 10% of wild type activity
I86F
the mutant shows about 16% of wild type activity
Q18N
the mutant shows about 20% of wild type activity
R179A
R179K
the mutant shows about 45% of wild type activity
S37A
the mutant shows about 85% of wild type activity
Y175A
the mutant shows about 10% of wild type activity
Y175F
the mutant shows about 9% of wild type activity
Y181A
the mutant shows about 7% of wild type activity
Y181F
the mutant shows about 5% of wild type activity
Y19F
the mutant shows about 30% of wild type activity
Y27A
the mutant shows about 5% of wild type activity
Y27F
the mutant shows about 8% of wild type activity
additional information