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1.8.99.B1: protein-disulfide reductase (acceptor)

This is an abbreviated version!
For detailed information about protein-disulfide reductase (acceptor), go to the full flat file.

Word Map on EC 1.8.99.B1

Reaction

protein-dithiol
+
oxidized acceptor
=
protein-disulfide
+
reduced acceptor

Synonyms

AaPDO, ApPDO, disulfide bond-forming enzyme, glutaredoxin related protein, PDO, PfPDO, protein disulfide oxidoreductase, SSO0192, SSO10610, Sso1120, SsPDO

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.99 With unknown physiological acceptors
                1.8.99.B1 protein-disulfide reductase (acceptor)

Crystallization

Crystallization on EC 1.8.99.B1 - protein-disulfide reductase (acceptor)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals are grown in the presence of 2 M ammonium sulfate, 2% (v/v) PEG 400, 0.1 M Hepes (pH 8). X-ray diffraction data are collected to 1.93 A resolution
crystal structure at 2.4 A resolution
purified recombinant enzyme, hanging-drop vapor-diffusion method, mixing of 0.001 ml of 5.5 mg/ml protein solution with 0.001 ml of reservoir solution containing 13% PEG 20000, and 0.1 M MES buffer, pH 6.0, method optimization, X-ray diffraction structure determination and analysis at 1.80 A resolution, molecular modelling
the X-ray crystallographic structure is solved at 1.80 A resolution