1.8.4.9: adenylyl-sulfate reductase (glutathione)

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For detailed information about adenylyl-sulfate reductase (glutathione), go to the full flat file.

Reaction

Synonyms

3'-phosphoadenosine-5'-phosphosulfate reductase homolog 19, 3'-phosphoadenosine-5'-phosphosulfate reductase homolog 26, 3'-phosphoadenosine-5'-phosphosulfate reductase homolog 43, 5'-adenylylsulfate reductase, 5-adenosinephosphosulphate reductase, adenosine 5'-phosphosulfate reductase, adenosine 5-phosphosulfate reductase, adenosine-5'-phosphosulfate reductase, adenosine-5'-phosphosulphate reductase, APR, APR1, APR1p, APR2, APS reductase, At1g62180, AtAPR1, CysH, EC 1.8.99.2, EiAPR, More, PAPS reductase homolog 19, PAPS reductase homolog 26, PAPS reductase homolog 43, plant-type 5'-adenylylsulfate reductase, PpAPR-B, Prh-19, Prh-26, Prh-43

ECTree

     1 Oxidoreductases

         1.8 Acting on a sulfur group of donors

             1.8.4 With a disulfide as acceptor

                1.8.4.9 adenylyl-sulfate reductase (glutathione)

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Results	in table
6	Activating Compound
17	AA Sequence
2	Application
1	CAS Registry Number
16	Cloned(Commentary)
13	Cofactor
3	Crystallization (Commentary)
3	Expression
13	General Information
2	General Stability
11	Inhibitors
4	Ki Value [mM]
25	KM Value [mM]
9	Localization
11	Metals/Ions
20	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
59	Organism
7	Pathway
1	PDB ID
6	pH Optimum
1	pH Range
7	Protein Variants
9	Purification (Commentary)
2	Reaction
3	Reaction Type
34	Reference
13	Source Tissue
23	Specific Activity [micromol/min/mg]
4	Storage Stability
71	Substrates and Products (Substrate)
9	Subunits
56	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
11	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.00075

3'-phosphoadenosine 5'-phosphosulfate

Physcomitrium patens

-

-

687585

0.012

3'-phosphoadenosine-5'-phosphosulfate

Mycobacterium tuberculosis

-

assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C

667703

8.4 - 11.3

5'-adenylyl sulfate

2 entries

0.153 - 56

5'-adenylylsulfate

4 entries

0.176

adenosine 5'-phosphosulfate

Physcomitrium patens

-

-

687585

10.1

dithiothreitol

Glycine max

-

30°C, pH 8.5

689431

8.4

glutathione

Glycine max

-

30°C, pH 8.5

689431

8.4

5'-adenylyl sulfate

Glycine max

-

30°C, pH 8.5, cosubstrate: dithiothreitol

689431

11.3

5'-adenylyl sulfate

Glycine max

-

30°C, pH 8.5, cosubstrate: glutathione

689431

0.153

5'-adenylylsulfate

Mycobacterium tuberculosis

-

assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C

667703

2.1

5'-adenylylsulfate

Catharanthus roseus

-

with glutathione as reductant

288642

3.4

5'-adenylylsulfate

Catharanthus roseus

-

with DTT as reductant

288642

56

5'-adenylylsulfate

Lemna minor

-

-

288644