1.8.4.8: phosphoadenylyl-sulfate reductase (thioredoxin)

This is an abbreviated version!
For detailed information about phosphoadenylyl-sulfate reductase (thioredoxin), go to the full flat file.

Reaction

Synonyms

3'-Phosphoadenosine 5'-phosphosulfate reductase, 3'-Phosphoadenylylsulfate reductase, 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase, 5'-adenylylsulfate reductase, Adenosine 3'-phosphate 5'-phosphosulfate reductase, adenosine 5'-phosphosulfate reductase, Adenylyl phosphosulfate reductase, APR, APS reductase, CysH1, EC 1.8.99.4, PAdoPS reductase, PAPS reductase, PAPS reductase, thioredoxin dependent, PAPS reductase, thioredoxin-dependent, PAPS sulfotransferase, Phospho-adenylylsulfate (PAPS) reductase, Phospho-adenylylsulfate reductase, Reductase, 3'-phosphoadenosine 5'-phosphosulfate, SNR, Thioredoxin: 3'-phospho-adenylylsulfate reductase, Thioredoxin:adenosine 3'-phosphate 5'-phosphosulfate reductase

ECTree

     1 Oxidoreductases

         1.8 Acting on a sulfur group of donors

             1.8.4 With a disulfide as acceptor

                1.8.4.8 phosphoadenylyl-sulfate reductase (thioredoxin)

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Results	in table
2	Activating Compound
4752	AA Sequence
1	Application
1	CAS Registry Number
8	Cloned(Commentary)
9	Cofactor
3	Crystallization (Commentary)
1	General Stability
6	Inhibitors
2	kcat/KM [mM/s]
30	KM Value [mM]
3	Metals/Ions
4	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
35	Organism
1	Oxidation Stability
7	Pathway
23	PDB ID
3	pH Optimum
5	Protein Variants
7	Purification (Commentary)
5	Reaction
2	Reaction Type
17	Reference
4	Specific Activity [micromol/min/mg]
43	Substrates and Products (Substrate)
6	Subunits
34	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
9	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.8.4.8 - phosphoadenylyl-sulfate reductase (thioredoxin)

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

3'-adenylyl-sulfate + thioredoxin

adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite

Bacillus subtilis

-

-

659441

-

-

?

3'-phosphoadenylyl sulfate + glutaredoxin 1

adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide

2 entries

3'-phosphoadenylyl sulfate + glutaredoxin Grx

adenosine 3',5'-bisphosphate + sulfite + glutaredoxin Grx disulfide

Escherichia coli

-

poplar glutaredoxin, 33% of the activity with thioredoxin Trx1

742273

-

-

?

3'-phosphoadenylyl sulfate + glutaredoxin Grx1

adenosine 3',5'-bisphosphate + sulfite + glutaredoxin Grx1 disulfide

Escherichia coli

-

Escherichia coli glutaredoxin, 70% of the activity with thioredoxin Trx1

742273

-

-

?

3'-phosphoadenylyl sulfate + glutaredoxin mutant 1C14S

adenosine 3',5'-bisphosphate + sulfite + glutaredoxin mutant 1C14S disulfide

Escherichia coli

-

-

659140

-

-

r

3'-phosphoadenylyl sulfate + thioredoxin

adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide

2 entries

3'-phosphoadenylyl sulfate + thioredoxin 1

adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide

2 entries

3'-phosphoadenylyl sulfate + thioredoxin 2

adenosine 3',5'-bisphosphate + sulfite + thioredoxin 2 disulfide

Escherichia coli

-

-

659140

-

-

r

3'-phosphoadenylyl sulfate + thioredoxin hTrx1

adenosine 3',5'-bisphosphate + sulfite + thioredoxin hTrx1 disulfide

Escherichia coli

-

human thioredoxin, 59% of the activity with thioredoxin Trx1

742273

-

-

?

3'-phosphoadenylyl sulfate + thioredoxin Trx1

adenosine 3',5'-bisphosphate + sulfite + thioredoxin Trx1 disulfide

Escherichia coli

-

Escherichia coli thioredoxin

742273

-

-

?

3'-phosphoadenylyl sulfate + thioredoxin Trx2

adenosine 3',5'-bisphosphate + sulfite + thioredoxin Trx2 disulfide

Escherichia coli

-

Escherichia coli thioredoxin, 38% of the activity with thioredoxin Trx1

742273

-

-

?

3'-phosphoadenylyl sulfate + thioredoxin TrxH1

adenosine 3',5'-bisphosphate + sulfite + thioredoxin TrxH1 disulfide

Escherichia coli

-

Arabidopsis thaliana thioredoxin, 18% of the activity with thioredoxin Trx1

742273

-

-

?

3'-phosphoadenylyl sulfate + thioredoxin TrxH2

adenosine 3',5'-bisphosphate + sulfite + thioredoxin TrxH2 disulfide

Escherichia coli

-

Arabidopsis thaliana thioredoxin, 23% of the activity with thioredoxin Trx1

742273

-

-

?

3'-phosphoadenylyl sulfate + thioredoxin TrxH3

adenosine 3',5'-bisphosphate + sulfite + thioredoxin TrxH3 disulfide

Escherichia coli

-

Arabidopsis thaliana thioredoxin, 154% of the activity with thioredoxin Trx1

742273

-

-

?

3'-phosphoadenylyl sulfate + thioredoxin TrxH4

adenosine 3',5'-bisphosphate + sulfite + thioredoxin TrxH4 disulfide

Escherichia coli

-

Arabidopsis thaliana thioredoxin, 45% of the activity with thioredoxin Trx1

742273

-

-

?

3'-phosphoadenylyl-sulfate + glutaredoxin

adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite

2 entries

3'-phosphoadenylyl-sulfate + thioredoxin

adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite

4 entries

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

?

2 entries

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite

5 entries

adenosine 5'-phosphosulfate + thioredoxin

AMP + sulfite + oxidized thioredoxin

Mycobacterium tuberculosis

-

-

712502

-

-

?

adenosine 5'-phosphosulfate + thioredoxin I

AMP + sulfite + oxidized thioredoxin I

Pseudomonas aeruginosa

-

-

698950

-

-

?

additional information

?

-

6 entries

3'-phosphoadenylyl sulfate + glutaredoxin 1

adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide

Bacillus subtilis

-

-

659441

-

-

r

3'-phosphoadenylyl sulfate + glutaredoxin 1

adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide

Escherichia coli

-

-

659140

-

-

r

3'-phosphoadenylyl sulfate + thioredoxin

adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide

Saccharomyces cerevisiae

-

thioredoxin from Escherichia coli

133775

-

-

r

3'-phosphoadenylyl sulfate + thioredoxin

adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide

Saccharomyces cerevisiae

-

thioredoxin from Saccharomyces cerevisiae

133775

-

-

r

3'-phosphoadenylyl sulfate + thioredoxin 1

adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide

Bacillus subtilis

-

-

659441

-

-

r

3'-phosphoadenylyl sulfate + thioredoxin 1

adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide

Escherichia coli

-

-

659140

-

-

r

3'-phosphoadenylyl-sulfate + glutaredoxin

adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite

Bacillus subtilis

-

-

659441

-

-

?

3'-phosphoadenylyl-sulfate + glutaredoxin

adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite

Escherichia coli

-

-

659140, 659214

-

-

?

3'-phosphoadenylyl-sulfate + thioredoxin

adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite

Bacillus subtilis

-

-

659441

-

-

?

3'-phosphoadenylyl-sulfate + thioredoxin

adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite

Escherichia coli

-

-

393953, 659140, 659214

-

-

?

3'-phosphoadenylyl-sulfate + thioredoxin

adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite

Escherichia coli

-

enzyme catalyzes the first reductive step in sulfate assimilation

659140

-

-

?

3'-phosphoadenylyl-sulfate + thioredoxin

adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite

Escherichia coli

-

thioredoxin m from spinach

393953

-

-

?

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

?

Escherichia coli

-

enzyme is involved in sulfur metabolism

133777

-

-

?

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

?

Escherichia coli

-

essential step in the biosynthesis of Cys in E. coli. Blocking of the enzyme inhibits cell growth

133776

-

-

?

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite

Escherichia coli

-

-

133776, 133777

-

-

?

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite

Escherichia coli

-

-

133778

-

133778

?

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite

Saccharomyces cerevisiae

-

-

133775

-

133775

?

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite

Synechococcus sp.

-

-

133779

-

-

?

5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin

adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite

Synechococcus sp. PCC 7942

-

-

133779

-

-

?

additional information

?

-

Escherichia coli

-

enzyme is essential for sulfate assimilation using phosphoadenosine 5'-phosphosulfate, not adenosine 5'-phosphosulfate, phylogenetic comparison of the 2 different groups, overview

393953

-

-

?

additional information

?

-

Escherichia coli

-

enzyme is involved in sulfate assimilation requiring reduced glutathione and glutaredoxins, redox regulation of the enzyme by glutathione and glutaredoxins, overview

659214

-

-

?

additional information

?

-

Escherichia coli

-

thioredoxin1 and glutaredoxin 1 are essential for sulfate reduction but not for ribonucleotide reduction

659140

-

-

?

additional information

?

-

Escherichia coli

-

cofactor specificity

659140

-

-

?

additional information

?

-

Escherichia coli

-

the redox potential does not determine specificity nor efficiency of the redoxins as reductant. The efficiency of PAPS reductase with various redoxins correlates strongly to the extent of a negative electric field of the redoxins reaching into the solvent outside the active site, and electrostatic and geometric complementary contact surfaces

742273

-

-

?

additional information

?

-

Pseudomonas aeruginosa

-

thioredoxin I mutant W31A shows no detectable activity, whereas W31F, K36E, and D61N are able to serve as electron donors for the APR-catalyzed reaction but with lower turnover numbers than that exhibited by the wild type thioredoxin I. The Km for thioredoxin mutant R73E is increased by 7.7fold compared with wild type thioredoxin I

698950

-

-

?