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1.8.1.9: thioredoxin-disulfide reductase

This is an abbreviated version!
For detailed information about thioredoxin-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.9

Reaction

thioredoxin
+
NADP+
=
thioredoxin disulfide
+
NADPH
+
H+

Synonyms

all0737, ApTR, At2g41680, AtNTRA, AtNTRB, bacillithiol disulfide reductase, bacillithiol-disulfide reductase, BBOV_I002190, Bdr, BSSB reductase, CeTR2, DmTR, DmTrxR, DmTrxR-1, EC 1.6.4.5, EhTRXR, ferredoxin:thioredoxin reductase, FTR, general stress protein 35, GSP35, HCOI_01258400, hemolysate thioredoxin reductase, hemolysate TR, hTrxR, HvNTR1, HvNTR2, L-TR, L-TrxR, More, MtNTRC, mTR3, multifunctional thioredoxin-glutathione reductase, NAD(P)H:paraquat oxidoreductase, NADP-dependent thioredoxin reductase, NADP-linked thioredoxin reductase, NADP-thioredoxin reductase, NADP-thioredoxin reductase C, NADPH thioredoxin reductase, NADPH thioredoxin reductase C, NADPH-dependent thioredoxin reductase, NADPH-dependent thioredoxin reductase 2, NADPH-dependent thioredoxin reductase C, NADPH-dependent thioredoxin reductase I, NADPH-dependent thioredoxin reductase-1, NADPH-dependent TRX reductase, NADPH-thioredoxin reductase, NADPH-thioredoxin reductase C, NADPH-Trx reductase, NADPH2:oxidized thioredoxin oxidoreductase, NAPDH-dependent BSSB reductase, NTR, Ntr1, NTR2, Ntr3, NTRA, NTRAB, NtrB, NTRC, PH0178, PH1426, PhRP, PhTrxR, protein-disulfide oxidoreductase, reductase, thioredoxin, SEP1, taTrxR, TGR, thioredoxin glutathione reductase, thioredoxin h reductase, thioredoxin reductase, thioredoxin reductase (NADPH), thioredoxin reductase 1, thioredoxin reductase 2, thioredoxin reductase-1, thioredoxin reductase1, TON_1603, TR, TR1, TR2, TR3, TRase, TrR, Trr1, Trr1p, TRR2, Trx1, Trx3, TrxB, TrxB1, TrxB2, TrxR, TrxR-1(cyto), TrxR-1(mito), TrxR1, TrxR2, TrxR3, TrxRB3, TRXRD, TrxRh1, TrxRh2, TrxT, Txnrd1, TXNRD1-v3, Txnrd2, TXNRD3, TxrR-1, YpdA

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.9 thioredoxin-disulfide reductase

Specific Activity

Specific Activity on EC 1.8.1.9 - thioredoxin-disulfide reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
TrxR-16 mutant K29R/H108Y/A119N/V478E
0.007
-
TrxR-16 mutant K29R/H108Y
0.016
-
TrxR-16 mutant K29R
0.025
-
TrxR-16, TrxR lacking the last 16 C-terminal amino acids
0.035
-
mutant U498C
0.044
-
substrate S-nitrosoglutathione, pH 7.8, 37°C
0.068
-
substrate sodium selenite, pH 7.8, 37°C
0.2
-
purified double mutant C535S/C88A as homodimer, insulin coupled assay
0.22
-
mutant H509Q, coupled assay with DTNB
0.495
-
substrate ebselen, pH 7.8, 37°C
0.5
-
activity with 5,5'-dithiobis(2-nitrobenzoic acid) and NADH, pH and temperature not specified in the publication
0.55
truncated mutant, pH 7.2, temperature not specified in the publication
0.595
-
purified enzyme
0.77
-
enzyme from cell-free extract, with NADPH, in 50 mM potassium phosphate buffer (pH 7.0), at 22°C
0.92
pH 7.2, temperature not specified in the publication
1.5
-
purified mutant C535A as heterodimer with the wild-type, insulin coupled assay
10.3
-
GSSG + NADPH
103
-
purified enzyme
13.9
-
mitochondrial isoform
14.1
using thioredoxin disulfide and NADPH as substrates, at 25°C
150.6
using 5,5'-dithiobis(2-nitrobenzoic acid) and NADPH as substrates, at 25°C
17.5
-
thioredoxin disulfide + NADPH
2.2
-
purified mutant C88A as heterodimer with the wild-type, insulin coupled assay
29.7
-
wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 20°C
3.9
-
purified wild-type enzyme, insulin coupled assay
31
-
DTNB coupled assay, purified enzyme
33.8
-
purified enzyme
35
-
purified enzyme, coupled assay with DTNB
36.4
using thioredoxin disulfide, insulin, and NADPH as substrates, at 25°C
367.7
-
after 478fold purification, with NADPH, in 50 mM potassium phosphate buffer (pH 7.0), at 22°C
4.6
-
purified enzyme, coupled assay with DTNB
42
-
purified enzyme, coupled assay with DTNB
5.1
-
mutant enzyme C145S, in 50 mM potassium phosphate (pH 7.0), at 20°C
5.5
-
mutant enzyme C142S, in 50 mM potassium phosphate (pH 7.0), at 20°C
5.6
-
mutant enzyme C145A, in 50 mM potassium phosphate (pH 7.0), at 20°C
58
-
purified enzyme
6
-
substrate 5,5'-dithio-bis(2-nitrobenzoic acid), pH 7.8, 37°C
6.4
-
mutant enzyme C142A, in 50 mM potassium phosphate (pH 7.0), at 20°C
67
-
purified enzyme
7.32
pH 7.2, temperature not specified in the publication
additional information