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1.8.1.7: glutathione-disulfide reductase

This is an abbreviated version!
For detailed information about glutathione-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.7

Reaction

2 glutathione +

NADP+
=
glutathione disulfide
 +
NADPH
 +
H+

Synonyms

At3g54660, EC 1.6.4.2, GLR, glutahione reductase, glutathione disulfide reductase, glutathione reductase, glutathione reductase (NADPH), glutathione reductase 3, glutathione reductase Glr1, glutathione S-reductase, glutathione: NADP(+) oxidoreductase, glutathione: NADP+ oxidoreductase, glutathione:NADP+ oxidoreductase, Gor, GOR1, GOR2, GR, GR1, GR2, Gr3, GRase, GRase-1, GRd, GSH reductase, GSHR, Gsr, GSR-1, GSSG reductase, HCOI_01258400, hGR, HvGR1, HvGR2, multifunctional thioredoxin-glutathione reductase, NADPH-glutathione reductase, NADPH-GSSG reductase, NADPH-reduced GR, NADPH: oxidized glutathione oxidoreductase, NADPH:oxidized glutathione oxidoreductase, NADPH:oxidized-glutathione oxidoreductase, OBP29, PfGR, psgr, PtGR1.1, PtGR1.2, PtGR2, reductase, glutathione, SpGR, TaGR1, TaGR2, TGR, thioredoxin glutathione reductase, thioredoxin/glutathione reductase, TrxR3

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.7 glutathione-disulfide reductase

Engineering

Engineering on EC 1.8.1.7 - glutathione-disulfide reductase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S150F
mutant displays strong inhibition of root growth and severe defects in the root apical meristem, with catalyitc activity being reduced to about 50%. The mutant accumulates high levels of GSSG and exhibits increased glutathione oxidation. The exogenous application of GSH or of dithiothreitol can rescue the root phenotype of mutant S150F
A18E//N21W/R22N/N101D/K105D/R319A
-
mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.5fold decrease in turnover-number for trypanothione, 9.5fold decrease in KM-value for trypanothione
A18E/N21W/R22N
-
mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.6fold increase in turnover-number for trypanothione, 3fold decrease in KM-value for trypanothione
A18E/N21W/R22N/N101D/K105D
-
mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.5fold increase in turnover-number for trypanothione, 6.7fold decrease in KM-value for trypanothione
N101D/K105D
-
about 2fold increase in KM-value for GSSG, 1.2fold decrease in turnover-number. 1.6fold decrease in turnover-number for trypanothione, 1.15fold increase in KM-value for trypanothione
N21R
-
1.6fold increase in KM-value for GSSG, 1.4fold decrease in turnover-number for GSSG, 1.2fold decrease in turnover-number for trypanothione, 2.3fold increase in turnover number for trypanothione
A34E/R37W
glutathione reductase mutant, activity switches to trypanothione reductase, termed GRTR, 700fold more activity with trypanothione than with glutathione
Y114L
additional information