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1.8.1.4: dihydrolipoyl dehydrogenase

This is an abbreviated version!
For detailed information about dihydrolipoyl dehydrogenase, go to the full flat file.

Word Map on EC 1.8.1.4

Reaction

protein N6-(dihydrolipoyl)lysine
+
NAD+
=
protein N6-(lipoyl)lysine
+
NADH
+
H+

Synonyms

apicoplast E3, CDS, CIP50, coronin-interacting protein, dehydrogenase, lipoamide, dehydrolipoate dehydrogenase, DHLDH, DHLipDH, diaphorase, dihydrolipoamide dehydrogenase, dihydrolipoamide dehydrogenase E3, dihydrolipoamide:NAD+ oxidoreductase, dihydrolipoic dehydrogenase, dihydrolipomide dehydrogenase, dihydrolipoyl dehydrogenase, DLD, DLD1, Dld2, DLDH, DLDH dehydrogenase, DLDH diaphorase, DLDH2, DT-diaphorase, E3, E3 component, E3 component of 2-oxoglutarate dehydrogenase complex, E3 component of acetoin cleaving system, E3 component of alpha keto acid dehydrogenase complexes, E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes, E3 component of pyruvate complex, E3 lipoamide dehydrogenase, E3 protein component of 2-oxoacid dehydrogenase multienzyme complexes, E3 subunit of the alpha-ketoglutarate dehydrogenase complex, EC 1.6.4.3, Glycine cleavage system L protein, Glycine oxidation system L-factor, hDLDH, hE3, hLADH, L-protein, LAD, LADH, LDH, LDP-Glc, LDP-Val, LipDH, lipoamide dehydrogenase, lipoamide dehydrogenase (NADH), lipoamide dehydrogenase C, lipoamide dehydrogenase2, lipoamide oxidoreductase (NADH), lipoamide reductase, lipoamide-dehydrogenase-valine, lipoate dehydrogenase, lipoic acid dehydrogenase, lipoyl dehydrogenase, LPD, LPD-GLC, LPD-VAL, LPD1, LPD2, Lpd3, LpdA, LpdC, LpdG, LpdV, More, mtLPD2, NAD(P)H:lipoamide oxidoreductase, NADH dehydrogenase, NADH diaphorase, NADH:lipoamide oxidoreductase, nicotinamide adenine dinucleotide diaphorase, ORF-E3, pdhD, pdhL, PfaE3, rhDLDH, TAase, ubiquinone reductase

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.4 dihydrolipoyl dehydrogenase

Molecular Weight

Molecular Weight on EC 1.8.1.4 - dihydrolipoyl dehydrogenase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
equilibrium sedimentation
102000
105000
107000
blue native PAGE
110000
112000
115000
117000
-
gel filtration
118000
-
gel filtration
119000
120000
-
gel filtration
125000
-
LPD-Glc, gel filtration
127478
-
sequence calculation
128000 - 140000
-
gel filtration and analytical ultracentrifugation
18000
-
native protein using SDS-PAGE
24980
-
predicted from cDNA
28000
-
recombinant protein using SDS-PAGE, in good agreement with the calculated molecular weight of recombinant DiaA
28206
-
x * 28206, sequence analysis
28430
-
x * 28430, sequence analysis
43000
-
sequence analysis
45000
2 * 45400, sequence analysis, 2 * 45000, SDS-PAGE
45400
2 * 45400, sequence analysis, 2 * 45000, SDS-PAGE
46000
-
x * 46000, SDS-PAGE
49342
-
x * 49342, electrospray mass spectrometry
49690
-
x * 49690, calculation from nucleotide sequence
49757
-
x * 49757, mitochondrial enzyme, electrospray mass spectrometry
49912
2 * 49912, calculated from amino acid sequence
50000
50027
x * 50027, MW only of protein, flavin is reduced during analysis, electrospray MS analysis
50070
-
enzyme including the bound FAD
50216
-
2 * 50216, amino acid sequence calculation
51000
51274
-
x * 51274, calculation from nucleotide sequence
52000
52614
-
x * 52614, enzyme from chloroplast, electrospray mass spectrometry
53000
54000
55000
56000
57000
x * 57000, SDS-PAGE, His-tagged recombinant protein
57200
-
2 * 57200, mitochondrial enzyme, SDS-PAGE and gel filtration, 2 * 75600, apicoplast enzyme, SDS-PAGE and gel filtration
58000
59000
-
x * 59000, SDS-PAGE
60000
-
2 * 60000, SDS-PAGE
61000
-
x * 61000, SDS-PAGE
61600
-
full-length recombinant DLDH, sequence analysis
63489
-
x * 63489, calculated
66500
x * 66500, SDS-PAGE
75600
-
2 * 57200, mitochondrial enzyme, SDS-PAGE and gel filtration, 2 * 75600, apicoplast enzyme, SDS-PAGE and gel filtration
86000
-
native form of recombinant DiaA, gel filtration
88000
-
gel filtration
98000
-
gel filtration