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1.7.7.2: ferredoxin-nitrate reductase

This is an abbreviated version!
For detailed information about ferredoxin-nitrate reductase, go to the full flat file.

Word Map on EC 1.7.7.2

Reaction

nitrite
+
H2O
+
oxidized ferredoxin
=
nitrate
+
reduced ferredoxin
+ 2 H+

Synonyms

assimilatory ferredoxin-nitrate reductase, Assimilatory nitrate reductase, C452_13229, dissimilatory nitrate reductase, EC 1.7.99.4, Fd-dependent nitrate reductase, Fd-NAS, NaR, narB, NarGH, NAS, NasB, nitrate (ferredoxin) reductase, nitrate reductase, reductase, nitrate (ferredoxin), respiratory nitrate reductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.7 With an iron-sulfur protein as acceptor
                1.7.7.2 ferredoxin-nitrate reductase

Storage Stability

Storage Stability on EC 1.7.7.2 - ferredoxin-nitrate reductase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-19°C, 3 months, enzyme loses no activity
-
-20°C, 20% glycerol, several months, no loss of activity
-20°C, in 50 mM Tris-HCl buffer, pH 7.5, 0.5 M NaCl, 1 mM EDTA, 1 month, no loss of activity
-
4°C, 50 mM phosphate buffer, pH 7, 20% glycerol, 2 weeks, no loss of activity
-
4°C, 8 days, in the absence of NaCl, little if any loss of activity
-
4°C, activity decreases about 40% in 1 week when the crude extract is stored. At temperatures higher than 4°C, the activity depletion in the crude extract is 60–80%
4°C, several weeks, no loss of activity
4°C, the activity of the pure sample is stable for 2–3 weeks