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1.7.7.2: ferredoxin-nitrate reductase

This is an abbreviated version!
For detailed information about ferredoxin-nitrate reductase, go to the full flat file.

Word Map on EC 1.7.7.2

Reaction

nitrite
+
H2O
 +
oxidized ferredoxin
=
nitrate
 +
reduced ferredoxin
 + 2 H+

Synonyms

assimilatory ferredoxin-nitrate reductase, Assimilatory nitrate reductase, C452_13229, dissimilatory nitrate reductase, EC 1.7.99.4, Fd-dependent nitrate reductase, Fd-NAS, NaR, narB, NarGH, NAS, NasB, nitrate (ferredoxin) reductase, nitrate reductase, reductase, nitrate (ferredoxin), respiratory nitrate reductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.7 With an iron-sulfur protein as acceptor
                1.7.7.2 ferredoxin-nitrate reductase

General Stability

General Stability on EC 1.7.7.2 - ferredoxin-nitrate reductase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme requires high salt concentration for stability and activity
low ionic strength, 70 h, 0.002 mg protein/ml, 10 mM phosphate buffer or 50 mM Tris-HCl buffer, pH 7.5, unstable
-
NaCl stabilizes, after 43 h, with 2.7 M, 2.1 M or 0.9 M NaCl: 95% loss of activity, after 90 h, with 4.3 M NaCl: completely stable
-
the enzyme is most stable in the absence of salt
-