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1.7.3.3: factor-independent urate hydroxylase

This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.

Word Map on EC 1.7.3.3

Reaction

Urate
+
O2
+
H2O
=
5-hydroxyisourate
+
H2O2

Synonyms

AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.3 With oxygen as acceptor
                1.7.3.3 factor-independent urate hydroxylase

Storage Stability

Storage Stability on EC 1.7.3.3 - factor-independent urate hydroxylase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-12°C, 0.10 mol/l Tris/HCl buffer (pH 8.0), 8 months, less than 20% loss of activity, enzyme remains stable
-
-15°C, 100 mM borate buffer, pH 8.0, containing 100 mM or more ammonium sulfate
-
-20°C and 4°C, mutant enzyme I27C/N289C, 60 days, almost no loss of activity
-20°C, 72 h, no loss of activity
-20°C, purified enzyme, 72 h, 20% loss of activity
-
0-4°C, crystals in (NH4)2SO4 solution, 3 months, stable
-
37°C, mutant enzyme I27C/N289C, 15 days, almost no loss of activity
37°C, purified enzyme, 12 h and 48 h, 50% and 100% loss of activity
-
3°C, 0.15% sodium carbonate, several weeks, stable
-
4°C, 0.10 mol/l Tris/HCl buffer (pH 8.0), 10 weeks, less than 20% loss of activity
-
4°C, 100 mM Tris-HCl buffer, pH 9.5, 90% of activity after 5 days
yeast-like fungi
-
4°C, 20 mM phosphate buffer, pH 7.8, for at least 1 month
-
4°C, 7 days, 40% loss of activity
-
4°C, 72 h, no loss of activity
4°C, free uricase in borate buffer (pH 8.5), 28 days, 70% loss of activity
-
4°C, purified enzyme, 72 h, 20% loss of activity
-
4°C, several weeks
-
4°C, uricase in lipid vesicles in borate buffer (pH 8.5), 28 days, no loss of activity
-