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1.7.3.3: factor-independent urate hydroxylase

This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.

Word Map on EC 1.7.3.3

Reaction

Urate
+
O2
 +
H2O
=
5-hydroxyisourate
 +
H2O2

Synonyms

AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.3 With oxygen as acceptor
                1.7.3.3 factor-independent urate hydroxylase

Molecular Weight

Molecular Weight on EC 1.7.3.3 - factor-independent urate hydroxylase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
102000
-
polyacrylamide disc electrophoresis
105000
-
gel filtration
109000
-
gel filtration
114000 - 128000
-
PAGE, gel filtration
115000 - 123000
-
PAGE, gel filtration
117000
-
gel filtration
120000
120000 - 122000
120000 - 140000
-
PAGE, gel filtration
124000
125000
128000
13500
-
gel filtration
135000
136300 - 141600
-
ultracentrifugation
137000
-
for the homotetramer, determined by neutron crystallographic analysis
144000
native enzyme, gel filtration
145000 - 150000
-
gel filtration
150000
gel filtration
151000
-
Sephadex G-200 gel filtration
18000
-
x * 39700, isoform UI, x * 3050, isoform UII, x * 55300, isoform UIII, x * 18000, isoform UIV, SDS-PAGE
230000
-
gel filtration
30000
-
alpha4, 4 * 30000, SDS-PAGE
32000
32000 - 35000
-
SDS-PAGE, gel filtration
32880
yeast-like fungi
-
deduced from nucleotide sequence of cDNA
33000
33270
-
determination of nucleotide sequence of cDNA and calculation of corresponding amino acid sequence
33750
-
4 * 33750, X-ray crystallography
33858
-
4 * 33858, calculation from nucleotide sequence
34000
34160
x * 34160, MALDI-TOF
35000
-
alpha2beta2, 2 * 35000 + 2 * 37000, SDS-PAGE
35050
-
deduced from nucleotide sequence of cDNA
35052
-
alpha4, 4 * 35052, calculated from deduced amino acid sequence
35060
-
MALDI-TOF mass spectrometry
35780
MALDI-TOF mass spectrometry
35790
calculated from amino acid sequence
36000
37000
39000
-
alpha2beta2, 2 * 36000 + 2 * 39000, SDS-PAGE
39700
-
x * 39700, isoform UI, x * 3050, isoform UII, x * 55300, isoform UIII, x * 18000, isoform UIV, SDS-PAGE
50000
-
gel filtration
55300
-
x * 39700, isoform UI, x * 3050, isoform UII, x * 55300, isoform UIII, x * 18000, isoform UIV, SDS-PAGE
58680
-
MALDI-TOF mass spectrometry
58900
-
calculated from amino acid sequence
60000
-
about 60000 Da, SDS-PAGE
62000
x * 62000, rasburicase, SDS-PAGE
64000
-
1 * 64000
68000
-
gel filtration
70000 - 76000
-
gel filtration
98000
-
x * 98000, uricase-MBP fusion protein