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1.7.2.3: trimethylamine-N-oxide reductase

This is an abbreviated version!
For detailed information about trimethylamine-N-oxide reductase, go to the full flat file.

Word Map on EC 1.7.2.3

Reaction

trimethylamine
+ 2 (ferricytochrome c)-subunit +
H2O
=
Trimethylamine N-oxide
+ 2 (ferrocytochrome c)-subunit + 2 H+

Synonyms

EC 1.6.6.9, More, reductase, trimethylamine N-oxide, TMAO reductase, TOR, TorA, TorD, torECA gene product, TorECAD, TorZ, trimethylamine N-oxide reductase, trimethylamine oxide reductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.2 With a cytochrome as acceptor
                1.7.2.3 trimethylamine-N-oxide reductase

Purification

Purification on EC 1.7.2.3 - trimethylamine-N-oxide reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap Chelating HP column chromatography
-
HiTrap Q FF column chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
-
of 2 enzyme forms, using ammonium sulfate treatment, ion exchange buffer treatment, column chromatography on DEAE-Sepharose CL6B and hydroxyapatite and gel filtration on Sephacryl S-300
-
recombinant His-tagged enzyme by nickel affinity and anion exchange chromatography
-
recombinant His-tagged enzyme from strain BL21(DE3) to near homogeneity
-
recombinant His-tagged monomeric, dimeric, and trimeric forms from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, and gel filtration for the the monomeric form
-
using ammonium sulfate treatment and chromatography on Sephacryl S-200 column and Mono-Q column
-
using heat treatment, ammonium sulfate precipitation and chromatography on Bio-Gel A-1.5m, DEAE-cellulose and reactive blue-agarose
-
using heat treatment, column chromatography on DEAE-cellulose, Sephadex G-200, DEAE-Sepharose CL-6B and hydroxyapatite
-
using ion exchange chromatography on DE52 column, chromatography on a Mono Q HR 16/10 column and preparative electrophoresis
using ion exchange chromatography on DE52, chromatography on a Mono Q HR 16/10 column and preparative electrophoresis
-
using polyethyleneimine to remove nucleic acids, ammonium sulfate treatment, column chromatography on DEAE-Sephacel and Bio-Gel A-5m, chromatofocusing and chromatography on Sephacryl-S-300 column
-