1.7.2.2: nitrite reductase (cytochrome; ammonia-forming)

This is an abbreviated version!
For detailed information about nitrite reductase (cytochrome; ammonia-forming), go to the full flat file.

Word Map on EC 1.7.2.2

1.7.2.2

six-electron

desulfovibrio

dissimilatory

succinogenes

wolinella

nitrosomonas

ammonification

thioalkalivibrio

nitratireducens

desulfuricans

chromatium

flavorubredoxin

deleyianum

menaquinol

hydrogenobacter

pentahaem

hildenborough

sulfurospirillum

ferrocytochrome

flavohemoglobins

cxxch

analysis

Reaction

+ 2 H2O + 6 ferricytochrome c =

+ 6 ferrocytochrome c + 7 H+

Synonyms

ammonia-forming cytochrome c nitrite reductase, ccNiR, CcuHao, CfHao, CmHao, cNiR, cytochrome c NiR, cytochrome c nitrite reductase, cytochrome c nitrite reductase complex, cytochrome c-552, epsilonHao, epsilonproteobacterial hydroxylamine oxidoreductase, Glov_1042, haoA, hexaheme c-type cytochrome, More, multihaem c NiR, multiheme cytochrome c nitrite reductase, multiheme nitrite reductase, NiR, NiR cytochrome c552, nirB, NirC, nitrite reductase, NpHao, NrfA, NrfA2NrfH complex, NrfHA, octahaem cytochrome c nitrite reductase, SCO2486, SCO2488, TvNiR, WS0969

ECTree

1 Oxidoreductases

1.7 Acting on other nitrogenous compounds as donors

1.7.2 With a cytochrome as acceptor

1.7.2.2 nitrite reductase (cytochrome; ammonia-forming)

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Results	in table
5	Activating Compound
8437	AA Sequence
4	Application
1	CAS Registry Number
10	Cloned(Commentary)
37	Cofactor
19	Crystallization (Commentary)
2	Expression
14	General Information
1	General Stability
30	Inhibitors
3	kcat/KM [mM/s]
3	Ki Value [mM]
41	KM Value [mM]
12	Localization
22	Metals/Ions
26	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
85	Organism
5	Pathway
74	PDB ID
13	pH Optimum
2	pH Range
26	Protein Variants
18	Purification (Commentary)
2	Reaction
7	Reaction Type
76	Reference
1	Source Tissue
39	Specific Activity [micromol/min/mg]
1	Storage Stability
119	Substrates and Products (Substrate)
20	Subunits
116	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
7	Temperature Stability [°C]
18	Turnover Number [1/s]

Purification

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Purification on EC 1.7.2.2 - nitrite reductase (cytochrome; ammonia-forming)

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5 entries

native enzyme 57.7fold by anion exchange chromatography and gel filtration

Thioalkalivibrio nitratireducens

Q5F2I3

696159

non-denaturing preparative polyacrylamide gel electrophoresis

Thioalkalivibrio nitratireducens

672400

purification by sepharose fast flow chromatography and gel filtration

Lacticaseibacillus casei

765367

Q-Sepharose HP column chromatography, Superdex S200 gel filtration and S-200 HR column chromatography

Desulfovibrio vulgaris

671148

recombinant MBP-tagged enzyme from Wolinella succinogenes strain DSM 1740 by affinity chromatography, cleavage of the MBP-tag by TEV protease

Nautilia profundicola

743303

recombinant protein, by Strep-tag II affinity chromatography followed by size-exclusion chromatography

Trichlorobacter lovleyi

B3E641

765066

recombinant wild-type and mutant enzymes from Escherichia coli strain JCB4083a by ammonium sulfate fractionation and anion exchange chromatography

Escherichia coli

P0ABK9

696256

recombinant wild-type and mutant MBP-tagged enzymes from Wolinella succinogenes strain DSM 1740 by affinity chromatography, cleavage of the MBP-tag by TEV protease

3 entries