1.7.1.4: nitrite reductase [NAD(P)H]
This is an abbreviated version!
For detailed information about nitrite reductase [NAD(P)H], go to the full flat file.
Word Map on EC 1.7.1.4
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1.7.1.4
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denitrify
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alcaligenes
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denitrification
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achromobacter
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cunirs
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cycloclastes
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dissimilatory
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pseudoazurins
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xylosoxidans
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1.6.6.4
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n2o
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cu-containing
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azurins
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acnir
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analysis
- 1.7.1.4
-
denitrify
- alcaligenes
-
denitrification
- achromobacter
- cunirs
- cycloclastes
-
dissimilatory
- pseudoazurins
- xylosoxidans
-
1.6.6.4
- n2o
-
cu-containing
- azurins
- acnir
- analysis
Reaction
+ 3 NAD(P)+ + 2 H2O = + 3 NAD(P)H + 5 H+
Synonyms
assimilitory nitrite reductase, copper-containing Nir, copper-containing nitrite reductase, EC 1.6.6.4, NAD(P)H:nitrite oxidoreductase, NADH-nitrite oxidoreductase, NADPH-nitrite reductase, NiR, NirK, nitrite reductase, nitrite reductase (reduced nicotinamide adenine dinucleotide (phosphate)), nitrite reductase [NAD(P)H2], NrfA reductase
ECTree
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Inhibitors
Inhibitors on EC 1.7.1.4 - nitrite reductase [NAD(P)H]
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1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate
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preincubation with NO2- completely protects
KCNS
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0.1 mM, 90% inhibition of NH2OH reductase activity, nitrite reductase activity is not affected
O2
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enzyme activity under anaerobic conditions is 2fold higher than under aerobic conditions
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1 mM, 40% inhibition, no inhibition if enzyme is preincubated with nitrite or NH2OH prior to adding the inhibitor
CN-
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1 mM, 45% inhibition, no inhibition if enzyme is preincubated with nitrite or NH2OH prior to adding the inhibitor
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1 mM, 64% inhibition after 5 min, reduced glutathione restores activity to 94%
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inactivation after preincubation, presence of nitrite protects from inactivation
NAD(P)H
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in vitro inactivation after preincubation for 5-10 min in the presence of FAD
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hydroxylamine reduction, substrate inhibition at low concentration, mixed inhibition vs. NADH
NAD+
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substrate inhibition at high concentration, required for full activity at low concentrations, maximum at 1 mM
nitrite
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substrate inhibition with increasing concentrations, complete inhibition with 50 mM nitrite
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1 mM, 79% inhibition after 5 min, dithiothreitol completely reverses activity, reduced glutathione, L-cysteine and 2-mercaptoethanol reverse activity to 86%, 64% and 82% respectively
p-chloromercuribenzoate
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0.02 mM, complete inactivation, partially prevented by 10 mM cysteine
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0.01 mM, 42% inhibition, 0.1 mM, complete inhibition
p-hydroxymercuribenzoate
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very potent inhibitor, inhibition can be reversed by either cysteine or glutathione
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intracellularly accumulated nitrite can inhibit nitrate uptake, addition of nitrite at concentrations above 5 mM is toxic and causes growth retardation
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additional information
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a progressive inhibition of activity is observed with increasing level of salinity
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