1.7.1.3: nitrate reductase (NADPH)

This is an abbreviated version!
For detailed information about nitrate reductase (NADPH), go to the full flat file.

Reaction

Synonyms

assimilatory NADPH-nitrate reductase, assimilatory NADPH:nitrate reductase, Assimilatory nitrate reductase, assimilatory reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase, EC 1.6.6.3, EC 1.7.99.4, MSMEG_2837, NADPH-dependent nitrate reductase, NADPH-nitrate reductase, NADPH2:nitrate oxidoreductase, NADPH:nitrate reductase, NADPH:NR, NaR1, narB, nit-3, nitrate reductase, nitrate reductase (NADPH), nitrate reductase (reduced nicotinamide adenine dinucleotide phosphate), nitrate reductase [NADPH], NR, triphosphopyridine nucleotide-nitrate reductase

ECTree

     1 Oxidoreductases

         1.7 Acting on other nitrogenous compounds as donors

             1.7.1 With NAD⁺ or NADP⁺ as acceptor

                1.7.1.3 nitrate reductase (NADPH)

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Results	in table
8	Activating Compound
1181	AA Sequence
6	Application
1	CAS Registry Number
9	Cloned(Commentary)
49	Cofactor
1	Crystallization (Commentary)
4	General Information
2	General Stability
41	Inhibitors
4	Ki Value [mM]
26	KM Value [mM]
1	Localization
27	Metals/Ions
23	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
3	Organic Solvent Stability
40	Organism
4	Pathway
18	pH Optimum
2	pH Range
1	pH Stability
20	Protein Variants
15	Purification (Commentary)
5	Reaction
3	Reaction Type
38	Reference
23	Source Tissue
26	Specific Activity [micromol/min/mg]
13	Storage Stability
65	Substrates and Products (Substrate)
22	Subunits
28	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Range [°C]
17	Temperature Stability [°C]
10	Turnover Number [1/s]

Purification

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Purification on EC 1.7.1.3 - nitrate reductase (NADPH)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

ammonium slufate precipitation, ion-exchange, gel filtration, hydroxylapatite column. FAD and EDTA essential in all buffers during purification

Aspergillus nidulans

-

395971

ammonium sulfate precipitation

Neurospora crassa

-

395974, 395975

gel filtration

Neurospora crassa

-

395977

immobilized metal affinity chromatography and SourceQ15 column anion exchange chromatography

Ogataea angusta

-

676411

ion-exchange, ammonium sulfate precipitation, gel filtration

Aspergillus nidulans

-

395968

metal-chelate affinity chromatography for his-tagged proteins, ammonium sulfate fractionation, affinity chromatography

Neurospora crassa

-

395976

native enzyme 70fold by ultrafiltration, anion exchange chromatography, and gel filtration

Fusarium oxysporum

-

741637

partially purification of wild-type and nit-3 mutant by streptomycin sulfate precipitation and ammonium sulfate fractionation, of nit-1 mutant by protamine sulfate fractionation, ammonium sulfate precipitation and gel filtration

Neurospora crassa

-

395980

partially purified

Neurospora crassa

-

395981

protamine sulfate and ammonium sulfate precipitation, hemoglobin-Sepharose column, Bio-Gel A, FAD-Sepharose affinity column. proteolysis during extensive purification

Neurospora crassa

-

395978

quick purification by immunoprecipitation with monospecific anti-nitrate reductase serum, stabilization during purification and protection of proteolytic cleavage by addition of phenylmethylsulphonyl fluoride

Aspergillus nidulans

-

395972

recombinant enzyme from Escherichia coli strains TP1000, RK5206, and RK5204 by a two-step affinity purification

Neurospora crassa

-

742843

salt fractionation, gel filtration , ion-exchange

Aspergillus nidulans

-

395979

streptomycin sulfate and ammonium sulfate precipitation, ion-exchange, gel filtration, isoelectric focusing, FAD-affinity

Neurospora crassa

-

395970