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1.7.1.15: nitrite reductase (NADH)

This is an abbreviated version!
For detailed information about nitrite reductase (NADH), go to the full flat file.

Word Map on EC 1.7.1.15

Reaction

NH3
+ 3 NAD+ + 2 H2O =
nitrite
+ 3 NADH + 5 H+

Synonyms

assimilatory NADH-nitrite reductase, assimilatory nitrite reductase, BMWSH_4089, EC 1.6.6.4, NADH-dependent nitrite oxidoreductase, NADH-dependent nitrite reductase, NADH-nitrite oxidoreductase, NADH-nitrite reductase, NADH:nitrite oxidoreductase, NasB, NasB protein, nasD, NasDE, nasE, NIA1, Nia2, NiR, nirB, NirBD, nirD, nitrite reductase (reduced nicotinamide adenine dinucleotide)

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.15 nitrite reductase (NADH)

Purification

Purification on EC 1.7.1.15 - nitrite reductase (NADH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation
DEAE-cellulose column chromatography
-
Ni-NTA column chromatography
purified in the presence of 1 mM NO2- and 0.01 mM FAD by ammonium sulfate precipitation, DEAE-cellulose column chromatography, DEAE-Sephadex gel filtration, and Sephadex G-25 gel filtration
-