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1.7.1.1: nitrate reductase (NADH)

This is an abbreviated version!
For detailed information about nitrate reductase (NADH), go to the full flat file.

Word Map on EC 1.7.1.1

Reaction

nitrite
+
NAD+
 +
H2O
=
nitrate
 +
NADH
 +
H+

Synonyms

assimilatory NADH:nitrate reductase, Assimilatory nitrate reductase, cytosolic NADH nitrate reductase, EC 1.6.6.1, EC 1.7.99.4, MSMEG_4206, MSMEI_4108, NADH-dependent nitrate reductase, NADH-Nar, NADH-nitrate reductase, NADH-NO3- reductase, NADH:nitrate oxidoreductase, NaR, NaR1, NasA, NasC, NIA1, Nia2, nitrate reductase, NR, NR1, NR2, Pden_4449, reductase, nitrate

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.1 nitrate reductase (NADH)

Purification

Purification on EC 1.7.1.1 - nitrate reductase (NADH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Ble Sepharose chromatography
-
by affinity chromatography using 5'AMP Sepharose and monoclonal antibodies
-
extracting strength of different detergents on membrane-bound isozyme
-
flavin domain
HiTrap Q column anion-exchange chromatography
-
immunoaffinity chromatography using a monoclonal antibody
-
mutant enzymes K741R, K741H, K714A, K741E, K741M, K741Q, K741P
-
NADH:Fe(III)-citrate reductase activity copurifies and has identical electrophoretic mobility
-
partial
recombinant enzyme expressed in Pichia pastoris
-
removal of nicked subunits by affinity chromatography
-
Sephadex G-25 gel filtration
-
SourceQ15 column chromatography and Ni-NTA column chromatography
-
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli
-
wild-type and nr1-mutant
-