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1.6.5.4: monodehydroascorbate reductase (NADH)

This is an abbreviated version!
For detailed information about monodehydroascorbate reductase (NADH), go to the full flat file.

Word Map on EC 1.6.5.4

Reaction

NADH
+
H+
+ 2 monodehydroascorbate =
NAD+
+ 2 ascorbate

Synonyms

12-oxophytodienoate reductase 3, AFR reductase, AFR-reductase, AFRR, ascorbate free radical reductase, ascorbate free-radical reductase, ascorbic free radical reductase, At1g63940, At3g09940, AtMDAR1, defensin, MDA, MDA reductase, MDAR, MDAR-OX, MDAR1, MDAR2, MDAR5, MDAsA reductase (NADPH), MDHA, MDHAR, MDHAR1, MDHAR2, MDHAR3, MDHAR6, metallothionein-like type 1 protein, monodehydroascorbate radical reductase, monodehydroascorbate reductase, monodehydroascorbate reductase 2, monodehydroascorbate reductase 4, More, MT-2, MT1K, NADH-semidehydroascorbate oxidoreductase, NADH:AFR oxidoreductase, NADH:ascorbate radical oxidoreductase, NADH:semidehydroascorbic acid oxidoreductase, NEC3, Nectarin III, OPR3, Os09g0567300, peroxisomal monodehydroascorbate reductase, PpMDHAR1, PpMDHAR2, PpMDHAR3, SDA reductase, semidehydroascorbate reductase, Solyc09g009390, SOR, SPD1, thioredoxin H2, Trx h2

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.5 With a quinone or similar compound as acceptor
                1.6.5.4 monodehydroascorbate reductase (NADH)

Storage Stability

Storage Stability on EC 1.6.5.4 - monodehydroascorbate reductase (NADH)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM Tris/HCl buffer, pH 7.4, 2 mM dithiothreitol, 25% glycerol, 1 month, stable
-
-80°C, buffer containing 10 mM potassium phosphate at pH 6.5 10 mM 2-mercaptoethanol and 0.1 mM EDTA, several months, no loss of activity
-
4°C, 4°C, in absence of dithiothreitol, complete loss of activity within 1 day
-
4°C, pH 8, 30 d, almost complete loss of activity, residual activity up to 38% when stored in presence of 1-10 mM MgSO4
-