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1.6.3.4: NADH oxidase (H2O-forming)

This is an abbreviated version!
For detailed information about NADH oxidase (H2O-forming), go to the full flat file.

Reaction

2 NADH + 2 H+ +

O2
= 2 NAD+ + 2 H2O

Synonyms

H2O-forming NADH oxidase, LrNox, NADH oxidase, nicotinamide adenine dinucleotide oxidase, NOX, Nox-2, NoxA, NoxE, SpNox, water-forming NADH oxidase, YODC

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.3 With oxygen as acceptor
                1.6.3.4 NADH oxidase (H2O-forming)

Cloned

Cloned on EC 1.6.3.4 - NADH oxidase (H2O-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the T7 promoter-based plasmid pET28a to give pET28a-SpNox and then heterologously overexpressed in Escherichia coli BL21 (DE3)
-
expressed in bdhA gene-disrupted Bacillus subtilis
-
expressed in Escherichia coli BL21(DE3) cells
expressed in pyruvate decarboxylase-deficient Saccharomyces cerevisiae strain SOS2
-
expressed in Saccharomyces cerevisiae JHY605 cells
expressed in Saccharomyces cerevisiae strain BY4741
expressed in Saccharomyces cerevisiae strain BY4741 and Clostridium acetobutylicum strain 428
expressed in Saccharomyces cerevisiae strain SR8
expression in Escherichia coli
-
expression in Saccharomyces cerevisiae V5
overexpressed in Escherichia coli BL21(DE3)
-
overexpression in Lactococcus lactis strains, plasmid vector pNZ8020 under the control of the Lactococcus lactis nisA promoter