1.6.3.4: NADH oxidase (H2O-forming)
This is an abbreviated version!
For detailed information about NADH oxidase (H2O-forming), go to the full flat file.
Reaction
2 NADH + 2 H+ + = 2 NAD+ + 2 H2O
Synonyms
H2O-forming NADH oxidase, LrNox, NADH oxidase, nicotinamide adenine dinucleotide oxidase, NOX, Nox-2, NoxA, NoxE, SpNox, water-forming NADH oxidase, YODC
ECTree
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Cloned
Cloned on EC 1.6.3.4 - NADH oxidase (H2O-forming)
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cloned into the T7 promoter-based plasmid pET28a to give pET28a-SpNox and then heterologously overexpressed in Escherichia coli BL21 (DE3)
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expressed in Escherichia coli BL21(DE3) cells
expressed in pyruvate decarboxylase-deficient Saccharomyces cerevisiae strain SOS2
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expressed in Saccharomyces cerevisiae strain BY4741 and Clostridium acetobutylicum strain 428
overexpression in Lactococcus lactis strains, plasmid vector pNZ8020 under the control of the Lactococcus lactis nisA promoter