1.6.3.2: NAD(P)H oxidase (H2O-forming)
This is an abbreviated version!
For detailed information about NAD(P)H oxidase (H2O-forming), go to the full flat file.
Reaction
2 NADPH
+
Synonyms
KOD1, NAD(P)H oxidase, NOX, NOXtp, TK1299
ECTree
1.6.3.2 NAD(P)H oxidase (H2O-forming)Advanced search results
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Substrates Products on EC 1.6.3.2 - NAD(P)H oxidase (H2O-forming)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 NAD(P)H + H+ + O2
NAD(P)+ + 2 H2O
in anaerobes, flavin-dependent NAD(P)H oxidases play an important role protecting organisms from oxidative stress
-
-
?
3 NADH + 3 H+ + 2 O2
3 NAD+ + H2O
approximately 75% H2O and 25% H2O is produced. Km value of the enzyme towards NADH and NADPH is almost the same whereas specific activity is higher with NADPH compared to NADH
-
-
?
3 NADPH + 3 H+ + 2 O2
3 NADP+ + H2O
approximately 87% H2O and 13% H2O2 is produced. Km value of the enzyme towards NADH and NADPH is almost the same whereas specific activity is higher with NADPH compared to NADH
-
-
?
2 NADH + H+ + O2
NAD+ + 2 H2O
NADPH is more efficient as electron donor compared to NADH
-
-
?
2 NADH + H+ + O2
NAD+ + 2 H2O
NADPH is more efficient as electron donor compared to NADH
-
-
?
2 NADH + H+ + O2
NAD+ + 2 H2O
reaction is catalyzed by the native dimeric protein under physiological conditions (low amounts of O2) to detoxify O2. Oxidative stress induced conformational change implicates a functional switch of the enzyme. The oxidized enzyme protein can accelerate the aggregation of partially unfolded proteins. It can also bind nucleic acids and produce H2O2 to destroy DNA and RNA with the ultimate function of decreasing cell viability
-
-
?
2 NADH + H+ + O2
NAD+ + 2 H2O
predominantly converts O2 to H2O, but not to H2O2. When NADPH oxidation is performed at 80°C, approximately 2% of the NADPH supplied is used to produce H2O2. Cys45 participates in the direct four-electron reduction of O2 to H2O, and the Cys45 mutation alters the reaction to produce H2O2 instead of H2O. NADPH is more efficient as electron donor compared to NADH
-
-
?
2 NADH + H+ + O2
NAD+ + 2 H2O
reaction is catalyzed by the native dimeric protein under physiological conditions (low amounts of O2). Oxidative stress induced conformational change implicates a functional switch of the enzyme. The oxidized enzyme protein can accelerate the aggregation of partially unfolded proteins. It can also bind nucleic acids and produce H2O2 to destroy DNA and RNA with the ultimate function of decreasing cell viability
-
-
?
2 NADPH + 2 H+ + O2
2 NADP+ + 2 H2O
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
-
-
?
2 NADPH + 2 H+ + O2
2 NADP+ + 2 H2O
O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically
-
-
?
2 NADPH + 2 H+ + O2
2 NADP+ + 2 H2O
the enzyme may be involved in maintenance of an optimum intracellular redox ratio
-
-
?
2 NADPH + 2 H+ + O2
2 NADP+ + 2 H2O
O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically
-
-
?
2 NADPH + 2 H+ + O2
2 NADP+ + 2 H2O
reaction is catalyzed by the native dimeric protein under physiological conditions (low amounts of O2) to detoxify O2. Oxidative stress induced conformational change implicates a functional switch of the enzyme. The oxidized enzyme protein can accelerate the aggregation of partially unfolded proteins. It can also bind nucleic acids and produce H2O2 to destroy DNA and RNA with the ultimate function of decreasing cell viability
-
-
?
2 NADPH + 2 H+ + O2
2 NADP+ + 2 H2O
predominantly converts O2 to H2O. When NADPH oxidation is performed at 80°C, approximately 7% of the NADPH supplied is used to produce H2O2. Cys45 participates in the direct four-electron reduction of O2 to H2O, and the Cys45 mutation alters the reaction to produce H2O2 instead of H2O. NADPH is more efficient as electron donor compared to NADH
-
-
?
2 NADPH + 2 H+ + O2
2 NADP+ + 2 H2O
reaction is catalyzed by the native dimeric protein under physiological conditions (low amounts of O2). Oxidative stress induced conformational change implicates a functional switch of the enzyme. The oxidized enzyme protein can accelerate the aggregation of partially unfolded proteins. It can also bind nucleic acids and produce H2O2 to destroy DNA and RNA with the ultimate function of decreasing cell viability
-
-
?
NADPH + H+ + O2
NADP+ + H2O
the enzyme prefers NADPH over NADH
-
-
?
additional information
?
-
cytochrome c is not reduced under any assay conditions used
-
-
?
additional information
?
-
cytochrome c is not reduced under any assay conditions used
-
-
?
