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1.6.2.4: NADPH-hemoprotein reductase

This is an abbreviated version!
For detailed information about NADPH-hemoprotein reductase, go to the full flat file.

Word Map on EC 1.6.2.4

Reaction

NADPH
+
oxidized hemoprotein
=
NADP+
 +
reduced hemoprotein
 +
H+

Synonyms

107366505, aldehyde reductase (NADPH-dependent), ATR2, CPR, CPR I, CPR-X1, CPR-X2, CPR1, CPR2, CYPOR, cytochrome c reductase (reduced nicotinamide adenine dinucleotide phosphate, NADPH, NADPH-dependent), cytochrome P450 NADPH reductase, cytochrome P450 oxidoreductase, cytochrome P450 reductase, cytochrome P450-diflavin reductase, dihydroxynicotinamide adenine dinucleotide phosphate-cytochrome c reductase, FAD-cytochrome c reductase, ferrihemprotein P450 reductase, flavocytochrome P450 BM3, NAD(P)H cytochrome P450 reductase, NADP-cytochrome c reductase, NADP-cytochrome P-450 reductase, NADP-cytochrome reductase, NADPH cytochrome P450 reductase, NADPH dependent cytochrome P450 reductase, NADPH P450 oxidoreductase, NADPH P450 reductase, NADPH-CPR, NADPH-CYP reductase, NADPH-CYP450 reductase, NADPH-cytochrome c oxidoreductase, NADPH-cytochrome c reductase, NADPH-cytochrome p-450 reductase, NADPH-cytochrome P450 (CYP) oxidoreductase, NADPH-cytochrome P450 oxidoreductase, NADPH-cytochrome P450 reductase, NADPH-cytochrome P450 reductase 2, NADPH-dependent cytochrome c reductase, NADPH-dependent cytochrome P450 reductase, NADPH-dependent P450 reductase, NADPH-ferricytochrome c oxidoreductase, NADPH-ferrihemoprotein reductase, NADPH-P450 reductase, NADPH: cytochrome P450 oxidoreductase, NADPH: cytochrome P450 reductase, NADPH:CYP oxidoreductase, NADPH:cytochrome P450 oxidoreductase, NADPH:cytochrome P450 reductase, NADPH:ferrihemoprotein oxidoreductase, NCP1, neotetrazolin reductase, NPR, NP_000932, P-450 reductase, P450 BM3, P450 oxidoreductase, P450 Red, P450 reductase, P450R, P450REd, POR, RedA, reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase, reductase, cytochrome c (reduced nicotinamide adenine dinucleotide phosphate), SbCPR2, TcCPR-A, TcCPR-B, TcCPR-C, TPNH-cytochrome c reductase, TPNH2 cytochrome c reductase, WsCPR1, WsCPR2

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.4 NADPH-hemoprotein reductase

Storage Stability

Storage Stability on EC 1.6.2.4 - NADPH-hemoprotein reductase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C or -20°C, 10 mM phosphate buffer, pH 7.5, several months
-
-15°C, more than 1 year
-
-20°C, 50 mM Tris/HCl, pH 7.25, 1 mM EDTA, 50% glycerol, enzyme stored for less than 1 month prior to use
-
-20°C, N2-atmosphere, several weeks
-
-20°C, several weeks
-20°C, under nitrogen, several weeks
-
-70°C, 0.15 mM potassium phosphate buffer, pH 7, 1 mM mercaptoethanol, 1 mM EDTA, 1 micromol FMN, 1 micromol FAD, 0.3% Mulgofen BC-720, 30% glycerol, several months
-
-75°C, months, 5 mM 2'-AMP
-
-78°C, 30 mM potassium phosphate buffer, pH 7.7, 0.1 mM EDTA, 20% glycerol, 0.4 mM PMSF
-
-80°C, 10 mM potassium phosphate, pH 7.4, 0.02% Emulgen 911, 0.002 mM flavinmononucleotide
-
-80°C, 20 mM phosphate buffer, pH 7.5, 20% glycerol
-
-80°C, 4 weeks
-
-80°C, 50 mM phosphate buffer, pH 7.4, 0.1 mM EDTA, 20% glycerol
-
-90°C or -20°C, 5-10% loss of activity, reactivation by FAD, 24 h
-
0-4°C, several months
-
0°C, some days
-
4°C or room temperature, at both low and high ionic strength, in the presence or absence of NADP, FAD-depleted enzyme
-
expressed in E. coli, -80°C
-