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0.0098
1,4-Naphthoquinone
-
pH 7.8, 37°C
0.0026
2-hydroxyestradiol
-
pH 7.8, 37°C
0.077
2-methyl-1,4-naphthoquinone
-
pH 7.8, 37°C
0.0057
9,10-phenanthrenequinone
-
pH 7.8, 37°C
0.63
benzamidoxime
-
pH 7.4
0.00114 - 0.042
cytochrome b5
-
0.36
dapsone hydroxylamine
-
pH 7.4
0.0006 - 4.35
ferricyanide
0.01 - 0.013
ferricytochome b5
-
0.000007 - 14
ferricytochrome b5
0.00029 - 0.007
ferricytochrome c
0.001 - 0.107
ferrocytochrome b5
0.008
methemoglobin-ferrocyanide complex
-
-
-
0.25
N-hydroxy-2-amino-1-methyl-6-phenylimidazol[4,5-b]pyridine
-
pH 7.4
0.22
N-Hydroxy-4-aminobiphenyl
-
pH 7.4
0.27
Nitrofurantoin
-
pH 7.8, 37°C
0.328 - 0.83
oxidized 2,6-dichlorophenolindophenol
0.36
sulfamethoxazole hydroxylamine
-
pH 7.4
0.025 - 0.089
testosterone
-
depending on phosphate concentration
additional information
additional information
-
detailed analysis of biphasic rate of reduction of cytochrome b5 in membranes. The initial rapid phase is completed within 10 msec and over 90% of cytochrome b5 are reduced in 40 msec. Evaluation of data in terms of two-dimensional random walk model
-
0.00114
cytochrome b5
-
-
-
0.0025
cytochrome b5
-
G273 mutant enzyme
-
0.0028
cytochrome b5
-
-
-
0.003
cytochrome b5
K110Q mutant enzyme
-
0.0031
cytochrome b5
-
H49K mutant enzyme
-
0.004
cytochrome b5
K110E mutant enzyme
-
0.0088
cytochrome b5
-
native enzyme
-
0.0089
cytochrome b5
-
H49A mutant enzyme
-
0.009
cytochrome b5
K110R mutant enzyme
-
0.009
cytochrome b5
K110A mutant enzyme
-
0.0091
cytochrome b5
-
recombinant wild-type enzyme
-
0.01
cytochrome b5
recombinant wild-type enzyme
-
0.011
cytochrome b5
-
H49Y mutant enzyme
-
0.012
cytochrome b5
K110H mutant enzyme
-
0.015
cytochrome b5
-
-
-
0.017
cytochrome b5
-
DELTAF272 mutant enzyme
-
0.03
cytochrome b5
-
H49E mutant enzyme
-
0.03 - 0.04
cytochrome b5
-
lysosome- and detergent-solubilized enzyme
-
0.0362
cytochrome b5
-
L125A mutant enzyme
-
0.042
cytochrome b5
-
L41A mutant enzyme
-
0.0006
ferricyanide
-
H49K mutant enzyme
0.00104
ferricyanide
-
mutant P247L
0.00104
ferricyanide
-
mutant enzyme P247L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.0016
ferricyanide
pH 7.0, 25°C,T66V mutant enzyme
0.0022
ferricyanide
-
H49E mutant enzyme
0.0022
ferricyanide
pH 7.0, 25°C,T66A mutant enzyme
0.0025
ferricyanide
-
native enzyme
0.0025
ferricyanide
pH 7.0, 25°C, wild type enzyme
0.0026
ferricyanide
-
H49Y mutant enzyme
0.0028
ferricyanide
-
recombinant wild-type enzyme
0.0028
ferricyanide
-
H49A mutant enzyme
0.0031
ferricyanide
pH 7.0, 25°C,T66S mutant enzyme
0.005
ferricyanide
K110R mutant enzyme
0.0052
ferricyanide
-
DELTAF272 mutant enzyme
0.0052
ferricyanide
-
G273 mutant enzyme
0.0058
ferricyanide
mutant Y93S, pH 7.0, 25°C
0.006
ferricyanide
-
wild-type
0.006
ferricyanide
recombinant wild-type enzyme
0.006
ferricyanide
mutant P275L, 25°C, pH 7.0
0.0066
ferricyanide
mutant Y93F, pH 7.0, 25°C
0.0068
ferricyanide
mutant Y93W, pH 7.0, 25°C
0.007
ferricyanide
-
recombinant enzyme
0.007
ferricyanide
-
pH 7.0, wild type enzyme
0.007
ferricyanide
wild-type, 25°C, pH 7.0
0.007
ferricyanide
mutant G179P, pH 7.0
0.007
ferricyanide
mutant G179V, pH 7.0
0.007
ferricyanide
-
mutant G75S
0.007
ferricyanide
-
mutant V252M
0.0071
ferricyanide
wild-type, pH 7.0, 25°C
0.00723
ferricyanide
-
wild type enzyme, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.0074
ferricyanide
mutant P92A, pH 7.0, 25°C
0.0078
ferricyanide
mutant P92S, pH 7.0, 25°C
0.008
ferricyanide
wild-type, pH 7.0
0.008
ferricyanide
-
cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein
0.008
ferricyanide
-
pH 7.0, 25°C
0.008
ferricyanide
L148P mutant enzyme, pH 7.0, 25°C
0.008
ferricyanide
P144L mutant enzyme, pH 7.0, 25°C
0.008
ferricyanide
P144L/L148P mutant enzyme, pH 7.0, 25°C
0.008
ferricyanide
-
pH 7.0, S127P mutant enzyme
0.008
ferricyanide
wild type enzyme, pH 7.0, 25°C
0.008
ferricyanide
mutant G179A, pH 7.0
0.008
ferricyanide
mutant G179T, pH 7.0
0.008
ferricyanide
-
mutant G75S/V252M
0.008
ferricyanide
mutant P92G, pH 7.0, 25°C
0.0083
ferricyanide
mutant Y93D, pH 7.0, 25°C
0.0083
ferricyanide
mutant Y93H, pH 7.0, 25°C
0.0086
ferricyanide
mutant Y93A, pH 7.0, 25°C
0.01096
ferricyanide
-
mutant P247A
0.01096
ferricyanide
-
mutant enzyme P247A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.0136
ferricyanide
-
in the presence of 2 mM Ca2+
0.01563
ferricyanide
-
mutant P248A
0.01563
ferricyanide
-
mutant enzyme P248A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.01568
ferricyanide
-
mutant P248L
0.01568
ferricyanide
-
mutant enzyme P248L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.02646
ferricyanide
-
mutant P249A
0.02646
ferricyanide
-
mutant enzyme P249A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.03309
ferricyanide
-
mutant P249L
0.03309
ferricyanide
-
mutant enzyme P249L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.05
ferricyanide
K110H mutant enzyme
0.345
ferricyanide
-
at pH 6.4 and 25°C
0.37
ferricyanide
K110E mutant enzyme
0.38
ferricyanide
K110A mutant enzyme
0.76
ferricyanide
K110Q mutant enzyme
0.01
ferricytochome b5
L148P mutant enzyme, pH 7.0, 25°C
-
0.012
ferricytochome b5
P144L mutant enzyme, pH 7.0, 25°C
-
0.013
ferricytochome b5
P144L/L148P mutant enzyme, pH 7.0, 25°C
-
0.013
ferricytochome b5
wild type enzyme, pH 7.0, 25°C
-
0.000007
ferricytochrome b5
-
-
0.000088
ferricytochrome b5
-
-
0.0008
ferricytochrome b5
-
-
0.0008
ferricytochrome b5
-
yeast cytochrome b5
0.00148
ferricytochrome b5
-
S99A mutant enzyme
0.0015
ferricytochrome b5
-
calf cytochrome b5
0.004
ferricytochrome b5
wild-type, 25°C, pH 7.0
0.005
ferricytochrome b5
-
Y65A mutant enzyme
0.0053
ferricytochrome b5
-
in the presence of 2 mM Ca2+
0.0062
ferricytochrome b5
-
Y65F mutant enzyme
0.0066
ferricytochrome b5
-
recombinant wild-type enzyme
0.0069
ferricytochrome b5
-
K97R mutant enzyme
0.00693
ferricytochrome b5
-
mutant P247L
0.0073
ferricytochrome b5
-
R63K mutant enzyme
0.009
ferricytochrome b5
-
-
0.0099
ferricytochrome b5
-
S99V mutant enzyme
0.01
ferricytochrome b5
mutant Y93D, pH 7.0, 25°C
0.01
ferricytochrome b5
mutant Y93H, pH 7.0, 25°C
0.0104
ferricytochrome b5
-
recombinant wild-type enzyme
0.011
ferricytochrome b5
-
recombinant enzyme
0.011
ferricytochrome b5
mutant Y93F, pH 7.0, 25°C
0.011
ferricytochrome b5
mutant Y93W, pH 7.0, 25°C
0.0118
ferricytochrome b5
-
S99T mutant enzyme
0.012
ferricytochrome b5
mutant P275L, 25°C, pH 7.0
0.012
ferricytochrome b5
mutant P92G, pH 7.0, 25°C
0.012
ferricytochrome b5
mutant P92S, pH 7.0, 25°C
0.012
ferricytochrome b5
mutant Y93S, pH 7.0, 25°C
0.013
ferricytochrome b5
-
-
0.013
ferricytochrome b5
-
wild-type
0.013
ferricytochrome b5
wild-type, pH 7.0, 25°C
0.013
ferricytochrome b5
pH 7.0, 25°C,T66S mutant enzyme
0.013
ferricytochrome b5
-
pH 7.0, wild type enzyme
0.013
ferricytochrome b5
-
mutant V252M
0.01353
ferricytochrome b5
-
wild type enzyme, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.014
ferricytochrome b5
-
0.014
ferricytochrome b5
-
pH 7.0, S127P mutant enzyme
0.014
ferricytochrome b5
-
mutant G75S
0.014
ferricytochrome b5
-
mutant G75S/V252M
0.014
ferricytochrome b5
mutant Y93A, pH 7.0, 25°C
0.0142
ferricytochrome b5
-
recombinant K110A mutant enzyme
0.0143
ferricytochrome b5
-
-
0.015
ferricytochrome b5
mutant P92A, pH 7.0, 25°C
0.0161
ferricytochrome b5
-
K97A mutant enzyme
0.0167
ferricytochrome b5
-
recombinant K110R mutant enzyme
0.01735
ferricytochrome b5
-
-
0.0185
ferricytochrome b5
-
R63Q mutant enzyme
0.02
ferricytochrome b5
-
enzyme from eythrocyte membrane
0.02
ferricytochrome b5
-
enzyme from erythrocyte membrane
0.02
ferricytochrome b5
pH 7.0, 25°C, wild type enzyme
0.0207
ferricytochrome b5
-
mutant enzyme P247A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.024
ferricytochrome b5
-
-
0.02407
ferricytochrome b5
-
mutant P247A
0.0285
ferricytochrome b5
-
recombinant K110M mutant enzyme
0.03434
ferricytochrome b5
-
mutant enzyme P248A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.0349
ferricytochrome b5
-
-
0.035
ferricytochrome b5
-
enzyme from liver microsome membrane, solubilized with Triton X-100
0.04
ferricytochrome b5
-
enzyme from eythrocyte cytosol
0.04148
ferricytochrome b5
-
mutant P248A
0.0421
ferricytochrome b5
-
R63A mutant enzyme
0.04481
ferricytochrome b5
-
mutant enzyme P249A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.045
ferricytochrome b5
-
enzyme from liver microsome membrane, cathepsin D solubilized
0.05238
ferricytochrome b5
-
mutant P249A
0.055
ferricytochrome b5
-
mutant P249L
0.08199
ferricytochrome b5
-
mutant P248L
0.111
ferricytochrome b5
pH 7.0, 25°C,T66V mutant enzyme
0.125
ferricytochrome b5
pH 7.0, 25°C,T66A mutant enzyme
14
ferricytochrome b5
-
-
0.00029
ferricytochrome c
-
soluble isoform, pH 7, 37°C
0.00042
ferricytochrome c
-
membrane-bound isoform, pH 7, 37°C
0.007
ferricytochrome c
-
cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein
0.001
ferrocytochrome b5
mutant G179P, pH 7.0
0.008
ferrocytochrome b5
mutant G179A, pH 7.0
0.012
ferrocytochrome b5
-
pH 7.0, 25°C
0.013
ferrocytochrome b5
wild-type, pH 7.0
0.043
ferrocytochrome b5
mutant G179T, pH 7.0
0.107
ferrocytochrome b5
mutant G179V, pH 7.0
0.00016
NADH
-
enzyme from erythrocyte
0.0003
NADH
-
H49E mutant enzyme
0.0004 - 0.0005
NADH
-
lysosome- and detergent-solubilized enzyme
0.0006
NADH
-
recombinant wild-type enzyme
0.0006
NADH
-
enzyme from erythrocyte membrane
0.0006 - 0.0007
NADH
-
enzyme from erythrocyte and liver
0.00064
NADH
-
G273 mutant enzyme
0.00084
NADH
-
DELTAF272 mutant enzyme
0.0015
NADH
-
recombinant wild-type enzyme
0.0016
NADH
-
native enzyme
0.0019
NADH
-
H49A mutant enzyme
0.0019
NADH
-
S99T mutant enzyme
0.0021
NADH
-
R63K mutant enzyme
0.0023
NADH
-
recombinant K110R mutant enzyme
0.0027
NADH
-
H49Y mutant enzyme
0.0028
NADH
pH 7.0, 25°C,T66S mutant enzyme
0.0028
NADH
pH 7.0, 25°C,T66V mutant enzyme
0.0028
NADH
-
at pH 7.4 and 37°C
0.0029
NADH
-
K97R mutant enzyme
0.0029
NADH
pH 7.0, 25°C,T66A mutant enzyme
0.003
NADH
-
Y65F mutant enzyme
0.003
NADH
F251Y mutant enzyme, pH 7.0, 25°C
0.003
NADH
-
pH 7.0, wild type enzyme with cytochrome b5 as substrate
0.0031
NADH
-
recombinant wild-type enzyme
0.0031
NADH
pH 7.0, 25°C, wild type enzyme
0.0038
NADH
mutant Y93S, pH 7.0, 25°C
0.0042
NADH
-
Y65A mutant enzyme
0.0044
NADH
-
K97A mutant enzyme
0.0048
NADH
mutant P92A, pH 7.0, 25°C
0.0049
NADH
mutant Y93D, pH 7.0, 25°C
0.005
NADH
D239S/F251Y mutant enzyme, pH 7.0, 25°C
0.006
NADH
-
recombinant enzyme
0.006
NADH
wild-type, pH 7.0
0.006
NADH
wild-type, pH 7.0, 25°C
0.006
NADH
D239E mutant enzyme, pH 7.0, 25°C
0.006
NADH
-
pH 7.0, wild type enzyme with ferricyanide as substrate
0.006
NADH
wild type enzyme, pH 7.0, 25°C
0.006
NADH
mutant P275L, cosubstrate ferricyanide, 25°C, pH 7.0
0.0065
NADH
mutant P92G, pH 7.0, 25°C
0.0067
NADH
mutant Y93F, pH 7.0, 25°C
0.007
NADH
-
pH 7.0, 25°C
0.0073
NADH
mutant Y93A, pH 7.0, 25°C
0.0077
NADH
mutant P92S, pH 7.0, 25°C
0.0088
NADH
mutant Y93H, pH 7.0, 25°C
0.0094
NADH
-
S99V mutant enzyme
0.01
NADH
-
H49K mutant enzyme
0.01097
NADH
-
ferricyanide as electron acceptor
0.01097
NADH
-
wild type enzyme, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.0112
NADH
-
in the presence of 2 mM Ca2+
0.012
NADH
F251R mutant enzyme, pH 7.0, 25°C
0.0135
NADH
-
mutant P248L, ferricyanide as electron acceptor
0.0135
NADH
-
mutant enzyme P248L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.0155
NADH
-
mutant P249L, ferricyanide as electron acceptor
0.0155
NADH
-
mutant enzyme P249L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.017
NADH
D239S mutant enzyme, pH 7.0, 25°C
0.017
NADH
-
mutant G75S/V252M
0.01713
NADH
-
mutant P247A, ferricyanide as electron acceptor
0.01713
NADH
-
mutant enzyme P247A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.021
NADH
-
R63Q mutant enzyme
0.02407
NADH
-
mutant P248A, ferricyanide as electron acceptor
0.02407
NADH
-
mutant enzyme P248A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.02467
NADH
-
mutant P247L, ferricyanide as electron acceptor
0.02467
NADH
-
mutant enzyme P247L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.025
NADH
-
pH 7.0, S127P mutant enzyme with cytochrome b5 as substrate
0.025
NADH
mutant G179A, pH 7.0
0.026
NADH
-
S99A mutant enzyme
0.02928
NADH
-
mutant P249A, ferricyanide as electron acceptor
0.02928
NADH
-
mutant enzyme P249A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
0.052
NADH
-
mutant V252M
0.054
NADH
mutant Y93W, pH 7.0, 25°C
0.055
NADH
-
pH 7.0, S127P mutant enzyme with ferricyanide as substrate
0.104
NADH
-
R63A mutant enzyme
0.119
NADH
D239T mutant enzyme, pH 7.0, 25°C
0.121
NADH
-
recombinant K110A mutant enzyme
0.224
NADH
D239S/F251R mutant enzyme, pH 7.0, 25°C
0.512
NADH
D239T/F251R mutant enzyme, pH 7.0, 25°C
0.595
NADH
mutant G179P, pH 7.0
0.662
NADH
mutant G179T, pH 7.0
0.672
NADH
-
recombinant K110M mutant enzyme
1.077
NADH
mutant G179V, pH 7.0
2.623
NADH
wild-type, cosubstrate ferricyanide, 25°C, pH 7.0
0.001
NADPH
-
cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein
0.009
NADPH
D239T mutant enzyme, pH 7.0, 25°C
0.022
NADPH
D239S/F251R mutant enzyme, pH 7.0, 25°C
0.025
NADPH
D239S/F251Y mutant enzyme, pH 7.0, 25°C
0.044
NADPH
D239T/F251R mutant enzyme, pH 7.0, 25°C
0.094
NADPH
D239E mutant enzyme, pH 7.0, 25°C
0.138
NADPH
F251R mutant enzyme, pH 7.0, 25°C
0.268
NADPH
D239S mutant enzyme, pH 7.0, 25°C
0.375
NADPH
mutant G179V, pH 7.0
0.507
NADPH
mutant G179T, pH 7.0
0.617
NADPH
F251Y mutant enzyme, pH 7.0, 25°C
0.924
NADPH
wild-type, pH 7.0
0.924
NADPH
wild type enzyme, pH 7.0, 25°C
1.36
NADPH
mutant G179A, pH 7.0
2.317
NADPH
mutant G179P, pH 7.0
0.328
oxidized 2,6-dichlorophenolindophenol
-
at pH 6.4 and 25°C
0.83
oxidized 2,6-dichlorophenolindophenol
-
-
1.6.2.2 cytochrome-b5 reductase
results (
results (
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