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1.6.2.2: cytochrome-b5 reductase

This is an abbreviated version!
For detailed information about cytochrome-b5 reductase, go to the full flat file.

Word Map on EC 1.6.2.2

Reaction

NADH
+ 2 ferricytochrome b5 =
NAD+
 +
H+
 + 2 ferrocytochrome b5

Synonyms

b5 plusb5R, b5/b5R, B5R, cb5r, CBR, CBR1, CBR1A, CBR1B, CyB5R, CYB5R2, CYB5R3, Cyb5R4, cyt b5r, cyt b5R protein, cytochrome b5 reductase, cytochrome b5 reductase 2, cytochrome b5 reductase 3, DIA1, diaphorase I, dihydronicotinamide adenine dinucleotide-cytochrome b5 reductase, HPO-19, methemoglobin reductase, NADH cytochrome b5 oxidoreductase, NADH cytochrome B5 reductase, NADH-b5R, NADH-cytochrome b 5 reductase, NADH-cytochrome b5 reductase, NADH-cytochrome b5 reductase 3, NADH-cytochrome-b5 reductase, NADH-cytochrome-b5 reductase 3, NADH-dependent cytochrome b5 reductase, NADH-ferricyanide reductase, NADH-ferricytochrome b5 oxidoreductase, NADH-ferricytochrome reductase, NADH: ferricytochrome b5 oxidoreductase, NADH:cytochrome b5 reductase, NADH:ferricytochrome b5 oxidoreductase, Ncb5or, P34/P32, P35, reduced nicotinamide adeninedinucleotide-cytochrome b5 reductase, reductase, cytochrome b5, T05H4.4

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.2 cytochrome-b5 reductase

General Stability

General Stability on EC 1.6.2.2 - cytochrome-b5 reductase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
0.2% phosphatidycholine liposomes or Triton X-100 stabilize
-
application of HPLC during purification causes inactivation
dithiothreitol is essential for stability
-
EDTA is essential for stability
-
enzyme from erythrocyte loses its activity in the absence of EDTA after 5 d at 4°C
-
freezing/thawing: 50% inactivation
-
hydrophilic domain is unfolded at approx. 1 M guanidinium hydrochloride whereas much higher concentrations are required for denaturation of the hydrophobic domain
-
inactivation caused by solubilization with detergents, activity restored by phosphatidylcholine
-
inactivation during dialysis is prevented by NADH
-
phosphate stabilizes
-
Triton X-100 stabilizes
-
unstable, 40% loss of activity at 0°C overnight, 1 mg/ml gelatin stabilizes, FAD protects against inactivation
-