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1.6.2.2: cytochrome-b5 reductase

This is an abbreviated version!
For detailed information about cytochrome-b5 reductase, go to the full flat file.

Word Map on EC 1.6.2.2

Reaction

NADH
+ 2 ferricytochrome b5 =
NAD+
 +
H+
 + 2 ferrocytochrome b5

Synonyms

b5 plusb5R, b5/b5R, B5R, cb5r, CBR, CBR1, CBR1A, CBR1B, CyB5R, CYB5R2, CYB5R3, Cyb5R4, cyt b5r, cyt b5R protein, cytochrome b5 reductase, cytochrome b5 reductase 2, cytochrome b5 reductase 3, DIA1, diaphorase I, dihydronicotinamide adenine dinucleotide-cytochrome b5 reductase, HPO-19, methemoglobin reductase, NADH cytochrome b5 oxidoreductase, NADH cytochrome B5 reductase, NADH-b5R, NADH-cytochrome b 5 reductase, NADH-cytochrome b5 reductase, NADH-cytochrome b5 reductase 3, NADH-cytochrome-b5 reductase, NADH-cytochrome-b5 reductase 3, NADH-dependent cytochrome b5 reductase, NADH-ferricyanide reductase, NADH-ferricytochrome b5 oxidoreductase, NADH-ferricytochrome reductase, NADH: ferricytochrome b5 oxidoreductase, NADH:cytochrome b5 reductase, NADH:ferricytochrome b5 oxidoreductase, Ncb5or, P34/P32, P35, reduced nicotinamide adeninedinucleotide-cytochrome b5 reductase, reductase, cytochrome b5, T05H4.4

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.2 cytochrome-b5 reductase

Cloned

Cloned on EC 1.6.2.2 - cytochrome-b5 reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
all mutants expressed in soluble form in Escherichia coli BL21
cloning of cDNA
-
cloning of cDNA, expression in Saccharomyces cerevisiae
enzyme coexpressed together with cytochrome b5 and CYP2E1or with CYP2E1 but without cytochrome b5 in Salmonella typhimurium strain 7108
-
expressed in COS-7 cells
expressed in Escherichia coli
expressed in Escherichia coli as alpha-thrombin-cleavable fusion protein
-
expressed in Escherichia coli as His-tag fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-RIL
expressed in Escherichia coli SoluBL21 cells
expressed in Mus musculus
-
expressed in Pichia pastoris
-
expression in Escherichia coli
expression in Saccharomaces cerevisiae
expression of a soluble fusion protein comprising a b-type cytochrome containing domain and a FAD-containing domain
-
expression of wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzymes in Escherichia coli
-
expression of wild-type, K110A, K110M and K110R mutant enzymes in Escherichia coli
-
expression of wild-type, K110R, K110H, K110A, K110E and K110Q mutant enzymes in Escherichia coli
expression of wild-type, K41A and K125A mutant enzyme in Escherichia coli
-
expression of wild-type, K97A, K97R, S99A, S99T, S99V, R63A, R63Q, R63K, Y65A and Y65F mutant enzymes in Escherichia coli
-
heterologous expression in Escherichia coli or in yeast cells via different expression vectors, in Aspergillus oryzae using pNGA142, Salmonella typhimurium using pIN, in Spodoptera frugiperda Sf9 insect cells using pFASTBAC baculovirus vectors, in cell-free systems, in plant cells using pRT, in lymphoid cells using Epstein-Barr virus, and in CHO cells using SV40 transfection method
-
heterologous expression in Saccharomyces cerevisiae
-
mutant enzymes expressed in Escherichia coli BL21(DE3)-RIL as His-tag fusion proteins
soluble domain, His-tag
-
wild type and mutant enzyme expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL
-