1.5.99.15: dihydromethanopterin reductase (acceptor)
This is an abbreviated version!
For detailed information about dihydromethanopterin reductase (acceptor), go to the full flat file.
Reaction
Synonyms
AfpA, archaeal-flavoprotein-like flavoprotein, Bxe_B2440, dihydromethanopterin reductase, Dmr, DmrB, DmrX, H2MPT reductase, methylene H4MPT dehydrogenase B, MJ0208, MM1854, MtdB
ECTree
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Purification
Purification on EC 1.5.99.15 - dihydromethanopterin reductase (acceptor)
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recombinant His6-tagged enzyme from Escherichia col strain BE100 [BL21(DE3) RIL] in an anaerobic chamber by heat treatement at 65°C for 5 min, the supernatant is a second time heat treated at 85°C for 15 min,
recombinant His6-tagged enzyme from Escherichia coli strain BE100 [BL21(DE3) RIL] in an anaerobic chamber by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant N-terminally His6 -tagged enzyme from Escherichia coli strain BL21DE3 RIL cell-free extract by nickel affinity chromatography and gel filtration