1.5.99.13: D-proline dehydrogenase
This is an abbreviated version!
For detailed information about D-proline dehydrogenase, go to the full flat file.
Reaction
Synonyms
D-Pro dehydrogenase, D-Pro DH, D-proline dehydrogenase, dye-dependent proline dehydrogenase, dye-linked D-proline dehydrogenase, PDH
ECTree
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Application
Application on EC 1.5.99.13 - D-proline dehydrogenase
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analysis
energy production
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a bio-anode using L-glutamate as the fuel is constructed. To oxidize L-glutamate at the anode, glutamate dehydrogenase, derived from Pyrobaculum islandicum, and proline dehydrogenase derived from Pyrococcus horikoshii, are immobilized for a two-enzyme conjugate enzymatic and redox reaction. To achieve an efficient enzyme reaction and electron transfer, the immobilization ratio of proline dehydrogenase to glutamate dehydrogenase is controlled by varying the molar ratios of dithiobis succinimidyl undecanoate and nitrilotriacetic acid dihydrochloride
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an amperometric D-amino acid biosensor is prepared with a thermostable D-proline dehydrogenase and a carbon nanotube-ionic liquid gel
analysis
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development of a D-amino acids electrochemical sensor based on immobilization of thermostable D-proline dehydrogenase within agar gel membrane. The D-Pro DH/agar immobilized electrode is applied to determination of D-amino acids in a human urine sample