1.5.8.4: dimethylglycine dehydrogenase

This is an abbreviated version!
For detailed information about dimethylglycine dehydrogenase, go to the full flat file.

Word Map on EC 1.5.8.4

1.5.8.4

sarcosine

fad

flavinylated

folate-binding

wagner

medicine

diagnostics

Reaction

Synonyms

Csal_0990, DdhC, DMGDH, EC 1.5.99.2, Me2GlyDH, mMe2GlyDH, N,N-dimethylglycine oxidase, pMe2GlyDH

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.8 With a flavin or flavoprotein as acceptor

1.5.8.4 dimethylglycine dehydrogenase

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Results	in table
264	AA Sequence
4	Application
1	CAS Registry Number
8	Cloned(Commentary)
32	Cofactor
6	Crystallization (Commentary)
43	Disease/ Diagnostics
3	Expression
7	General Information
5	Inhibitors
12	kcat/KM [mM/s]
22	KM Value [mM]
20	Localization
5	Metals/Ions
9	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
32	Organism
5	Pathway
6	PDB ID
5	pH Optimum
4	Protein Variants
10	Purification (Commentary)
1	Reaction
4	Reaction Type
28	Reference
1	Renatured (Commentary)
27	Source Tissue
8	Specific Activity [micromol/min/mg]
2	Storage Stability
47	Substrates and Products (Substrate)
7	Subunits
15	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
5	Temperature Stability [°C]
18	Turnover Number [1/s]

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Cloned on EC 1.5.8.4 - dimethylglycine dehydrogenase

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development of an intracellular, heterologous expression system in Komagataella phaffii (formerly known as Pichia pastoris). Initial attempts to express the gene in Escherichia coli (BL21 DE3) yielded largely insoluble protein. The H109R variant is expressed in lower amounts compared to the wild-type enzyme

Homo sapiens

Q9UI17

742499

expressed in Escherichia coli BL21(DE3) cells as a C-terminally 6-His-tagged fusion protein which is predominantly in its apo form

Rattus norvegicus

Q63342

687135

expressed in Komagataella phaffii KM71H cells

Homo sapiens

Q9UI17

742499

expression in Escherichia coli

2 entries

expression in Komagataella phaffii, i.e. Pichia pastoris

Homo sapiens

Q9UI17

742499

overexpression in Escherichia coli strain JM109 as N-terminally His-tagged enzyme being equally distributed in the soluble fraction and the insoluble inclusion body fraction

Rattus norvegicus

657277

screening of a cDNA library prepared in Escherichia coli from rat liver polyA RNA in the plasmid vector peD-X

Rattus norvegicus

Q63342

392524