1.5.7.1: methylenetetrahydrofolate reductase (ferredoxin)
This is an abbreviated version!
For detailed information about methylenetetrahydrofolate reductase (ferredoxin), go to the full flat file.
Reaction
Synonyms
5,10-methylenetetrahydrofolate reductase, Fdred:methylene-THF oxidoreductase, metF, methylene-H4F reductase, methylene-tetrahydrofolate reductase, methylenetetrahydrofolate reductase, MetVF-type methylenetetrahydrofolate reductase, MetVF-type MTHFR, MTHFR
ECTree
1.5.7.1 methylenetetrahydrofolate reductase (ferredoxin)Advanced search results
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Substrates Products on EC 1.5.7.1 - methylenetetrahydrofolate reductase (ferredoxin)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + FADH2
5,10-methylenetetrahydrofolate + FAD
-
-
-
-
?
5,10-methylenetetrahydrofolate + reduced ferredoxin
5-methyltetrahydrofolate + ferredoxin
-
-
-
-
?
5-methyltetrahydrofolate + 2 benzyl viologen
5,10-methylenetetrahydrofolate + 2 reduced benzyl viologen + 2 H+
5-methyltetrahydrofolate + 2 oxidized ferredoxin
5,10-methylenetetrahydrofolate + 2 reduced ferredoxin + 2 H+
-
-
-
-
r
5-methyltetrahydrofolate + benzyl viologen
5,10-methylenetetrahydrofolate + reduced benzyl viologen
5-methyltetrahydrofolate + FAD
5,10-methylenetetrahydrofolate + FADH2
-
11% of the activity with methylene blue
-
-
?
5-methyltetrahydrofolate + menadione
5,10-methylenetetrahydrofolate + reduced menadiol
-
25% of the activity with methylene blue
-
-
?
5-methyltetrahydrofolate + rubredoxin
5,10-methylenetetrahydrofolate + reduced rubredoxin
-
11% of the activity with methylene blue
-
-
?
5-methyltetrahydrofolate + 2 benzyl viologen
5,10-methylenetetrahydrofolate + 2 reduced benzyl viologen + 2 H+
-
-
-
-
r
5-methyltetrahydrofolate + 2 benzyl viologen
5,10-methylenetetrahydrofolate + 2 reduced benzyl viologen + 2 H+
-
-
-
-
r
5-methyltetrahydrofolate + benzyl viologen
5,10-methylenetetrahydrofolate + reduced benzyl viologen
-
-
-
-
?
5-methyltetrahydrofolate + benzyl viologen
5,10-methylenetetrahydrofolate + reduced benzyl viologen
-
11% of the activity with methylene blue
-
-
?
additional information
?
-
-
no activity with pyridine nucleotides
-
-
?
additional information
?
-
-
reduced ferredoxin cannot be the physiological electron donor in vivo, since growth by acetogenesis from H2 + CO2 has a negative ATP yield. Reduced methyl viologen is used as electron donor for following the MVred:methylene-THF oxidoreductase activity during the purification procedure. With ethanol as product and Fdred as electron donor for the MetVF-type MTHFR, the ATP yield becomes slightly positive (0.14 ATP/ethanol). But since only minor amounts of ethanol are produced, this does not result in an overall positive ATP yield
-
-
-
additional information
?
-
-
the enzyme catalyzes reduction of methylenetetrahydrofolate with reduced benzyl viologen but not with NAD(P)H in either the absence or presence of oxidized ferredoxin. The enzyme also catalyzes the reversible reduction of benzyl viologen with NADH (diaphorase activity) at 48 U/mg
-
-
?
additional information
?
-
-
the enzyme catalyzes reduction of methylenetetrahydrofolate with reduced benzyl viologen but not with NAD(P)H in either the absence or presence of oxidized ferredoxin. The enzyme also catalyzes the reversible reduction of benzyl viologen with NADH (diaphorase activity) at 48 U/mg
-
-
?
