1.5.7.1: methylenetetrahydrofolate reductase (ferredoxin)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate reductase (ferredoxin), go to the full flat file.

Reaction

+ 2 H+

Synonyms

5,10-methylenetetrahydrofolate reductase, Fdred:methylene-THF oxidoreductase, metF, methylene-H4F reductase, methylene-tetrahydrofolate reductase, methylenetetrahydrofolate reductase, MetVF-type methylenetetrahydrofolate reductase, MetVF-type MTHFR, MTHFR

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.7 With an iron-sulfur protein as acceptor

1.5.7.1 methylenetetrahydrofolate reductase (ferredoxin)

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
1	Activating Compound
1	CAS Registry Number
1	Cloned(Commentary)
6	Cofactor
3	General Information
1	Inhibitors
4	KM Value [mM]
3	Localization
5	Metals/Ions
5	Molecular Weight [Da]
4	Organism
1	Oxidation Stability
5	Pathway
3	pH Optimum
2	pH Stability
3	Purification (Commentary)
1	Reaction
4	Reference
2	Source Tissue
3	Specific Activity [micromol/min/mg]
1	Storage Stability
14	Substrates and Products (Substrate)
4	Subunits
14	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 1.5.7.1 - methylenetetrahydrofolate reductase (ferredoxin)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Purification (commentary) Search

Clostridium formicaceticum

437714

native enzyme 57.6fold from cytoplasm by anion exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, and gel filtration, to homogeneity

Clostridium ljungdahlii

764936

native enzyme aerobically 40fold by ultracentrifugation, anion exchange chromatography, gel filtration, ultrafiltration, hydroxyapatite chromatography, followed by again ultrafiltration and gel filtration, enzyme MetF copurifies with the other five proteins encoded in the unit in a hexaheteromeric complex with an apparent molecular mass in the 320-kDa range. Recombinant Strep-tagged enzyme MetF anaerobically from Escherichia coli strain C41(DE3) by avidine affinity chromatography and ultrafiltration

Moorella thermoacetica

742782