1.5.1.5: methylenetetrahydrofolate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

5, 10-methylenetetrahydrofolate dehydrogenase, 5,10-CH2-THF dehydrogenase, 5,10-mehtylene tetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, bmMTHFD, CH2-THF dehydrogenase, Cpe FolD, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH1, FolD, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, MIS1, mitochondrial methylenetetrahydrofolate dehydrogenase, MTHFD, MTHFD1, MTHFD1 protein, MTHFD2, MTHFD2 protein, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase, NADP-dependent 5,10-methylene-THF dehydrogenase

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.1 With NAD⁺ or NADP⁺ as acceptor

                1.5.1.5 methylenetetrahydrofolate dehydrogenase (NADP+)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
6	Activating Compound
45268	AA Sequence
3	Application
1	CAS Registry Number
21	Cloned(Commentary)
64	Cofactor
5	Crystallization (Commentary)
236	Disease/ Diagnostics
1	Expression
20	General Information
10	General Stability
14	IC50 Value
56	Inhibitors
20	kcat/KM [mM/s]
9	Ki Value [mM]
82	KM Value [mM]
22	Localization
14	Metals/Ions
30	Molecular Weight [Da]
58	Natural Substrates/ Products (Substrates)
421	Organism
16	Pathway
72	PDB ID
13	pH Optimum
3	pH Range
16	Protein Variants
36	Purification (Commentary)
1	Reaction
6	Reaction Type
66	Reference
57	Source Tissue
34	Specific Activity [micromol/min/mg]
13	Storage Stability
91	Substrates and Products (Substrate)
19	Subunits
73	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Stability [°C]
28	Turnover Number [1/s]

Storage Stability

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Storage Stability on EC 1.5.1.5 - methylenetetrahydrofolate dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Storage Stability Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-17°C, partially purified enzyme after second ammonium sulphate fractionation stable for 2 weeks, 1 mM mercaptoethanol,

Bos taurus

-

392316

-20°C, 0.1% bovine plasma albumin

Enterococcus faecium

-

392306

-20°C, 1 month, residual activity of 50%

Pisum sativum

-

209737

-20°C, purified MTHFD2L in 25 mM Tris-Cl, 10 mM 2-mercaptoethanol, 100mM KCl, and 50% v/v glycerol at pH 7.5. The purified enzyme retains about 75% of initial 5,10-CH2THF dehydrogenase activity after 1 week of storage

Rattus norvegicus

D3ZUA0

742856

-20°C, stable for at least 2 months at diluted protein concentration

Moorella thermoacetica

-

392311

-20°C, stable fot at least 2 months

Clostridium cylindrosporum

-

392310

0-4°C, after 1 month retains 75% of the methylenetetrahydrofolate dehydrogenase activity

Sus scrofa

-

392317

10% glycerol required to prevent loss of activity during storage

Leishmania major

-

700281

2-4°C, 10% loss of activity in 4 days

Pisum sativum

-

392326

4°C, in the presence of 25% glycerol, 1 month, residual activity of 85%

Pisum sativum

-

209737

4°C, stable for at least 2 months

Moorella thermoacetica

-

392311

4°C, stable for at least a month

Moorella thermoacetica

-

392309

5°C, stable for several weeks in ammonium sulfate at 50% saturation

Salmonella enterica subsp. enterica serovar Typhimurium

-

392312