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1.5.1.3: dihydrofolate reductase

This is an abbreviated version!
For detailed information about dihydrofolate reductase, go to the full flat file.

Word Map on EC 1.5.1.3

Reaction

5,6,7,8-tetrahydrofolate
+
NADP+
=
7,8-dihydrofolate
+
NADPH
+
H+

Synonyms

5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase, 7,8-dihydrofolate reductase, At2g16370, At4g34570, bifunctional dihydrofolate reductase-thymidylate synthase, bifunctional TS-DHFR, BmDHFR, dehydrogenase, tetrahydrofolate, DFR-TS, DFR1, DfrA, DfrB, DHFR, DHFR type IIIC, DHFR-TS, DHFR-TS1, DHFR-TS2, DHFR2, DHFRL1, DHFRLS, dihydrofolate reductase, dihydrofolate reductase-like, dihydrofolate reductase-thymidylate synthase, dihydrofolate reductase:thymidylate synthase, dihydrofolic acid reductase, dihydrofolic reductase, EC 1.5.1.4, ecDHFR, folA, folA3, folic acid reductase, folic reductase, FolM, hDHFR, hDHFR-1, hDHFR-2, HjDHFR, hvDHFR1, hvDHFR2, LAU_0427, LBRM_06_0830, mDHFR, mjDHFR, More, myDHFR, NADPH-dihydrofolate reductase, pcDHFR, PKNH_0509600, ppDHFR, pteridine reductase, pteridine reductase:dihydrofolate reductase, PTR2, R-plasmid-encoded dihydrofolate reductase, R67 DHFR, R67 dihydrofolate reductase, reductase, dihydrofolate, S3DHFR, Smdhfr, Smp 175230, spDHFR, svDHFR, tcptr1, tetrahydrofolate dehydrogenase, THY-1, THY-2, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase-dihydrofolate reductase, Trimethoprim resistance protein, TS-DHFR, WUBG_00817

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.3 dihydrofolate reductase

Specific Activity

Specific Activity on EC 1.5.1.3 - dihydrofolate reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0025
-
bifunctional enzyme DHFR-thymidylate synthase, substrate folate
0.005
liver cell mitochondria, pH 7.5, temperature not specified in the publication
0.006
liver cell mitochondria, pH 7.5, temperature not specified in the publication
0.009
kidney cell mitochondria, pH 7.5, temperature not specified in the publication
0.011
substrate dihydrosarcinapterin, pH 6.8, 23°C
0.041
substrate dihydrosarcinapterin, pH 6.8, 23°C
0.044
mitochondria of cancer cell line 4T1, pH 7.5, temperature not specified in the publication
0.058
-
leukemia cells
0.06
mutant L62F, pH not specified in the publication, temperature not specified in the publication
0.6 - 0.7
-
recombinant mutants S108V and S108M
0.75
-
methotrexate-sensitive strain
0.82
-
wild-type, 25°C, pH 7.0
0.85
-
methotrexate-resistant strain
1
-
recombinant mutants S108C and S108L
1.2
purified recombinant bifunctional enzyme DHFR-TS with Tsf tag, pH 7.4, 25°C from a TS-deficient Escherichia coli strain (thyA-)
1.51
wild-type, pH not specified in the publication, temperature not specified in the publication
1.7
-
purified recombinant enzyme
1.75
-
purified enzyme
10.4
-
strain PC 178
10.8
-
purified enzyme
103
-
purified enzyme
11
-
methotrexate resistant cells, L1210 (R), 25°C
11.6
-
amethopterin-resistant cells L1210/S
122.9
13
pH 7.0, 25°C, recombinant His-tagged enzyme
138.4
Halalkalibacterium halodurans
-
wild-type, pH 7.5, 37°C
14
-
chicken liver, 28°C
15.5
-
pH 7.0, 37°C
156.3
-
-
16.1
-
-
17
-
recombinant wild-type enzyme
17.5
-
purified enzyme
172
-
triple mutant L16M/L20M/L42M from plasmid
173
-
recombinant enzyme
18.5
substrate 7,8-dihydrofolate, pH 6.8, 23°C
2
-
recombinant mutant S108A
20.8
-
purified enzyme
200
-
purified enzyme
22.3
-
-
250
-
purified enzyme
27
-
methotrexate resistant cells, sarcoma 180 (AT/3000), 37°C
29.69
-
-
3.13
substrate 7,8-dihydrofolate, pH 6.8, 23°C
321
-
purified recombinant enzyme
35.8
-
-
4
-
recombinant mutant S108G
4.7
-
purified, recombinant enzyme
40
purified telluromethionine-containing enzyme
42
-
recombinant enzyme, partially purified, after refolding
44
purified selenomethionine-containing enzyme
45.9
-
wild-type enzyme from plasmid
46
-
purified enzyme
5
-
recombinant mutant S108Q
5.3
-
purified enzyme
5.59
-
strain PC 174
55
-
trimethoprim resistant, 30°C
56.5
-
purified enzyme
6
-
pH 7.0, 25°C
6.4
-
bifunctional enzyme DHFR-thymidylate synthase, substrate 7,8-dihydrofolate
60
-
human KB cells, 37°C
7.4
-
isozyme II
85
-
isozyme I
9
-
recombinant mutant S108N
90.2
-
double mutant L16M/L20M from plasmid
92.7
-
mutant L20M from plasmid
additional information