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1.5.1.3: dihydrofolate reductase

This is an abbreviated version!
For detailed information about dihydrofolate reductase, go to the full flat file.

Word Map on EC 1.5.1.3

Reaction

5,6,7,8-tetrahydrofolate
+
NADP+
=
7,8-dihydrofolate
 +
NADPH
 +
H+

Synonyms

5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase, 7,8-dihydrofolate reductase, At2g16370, At4g34570, bifunctional dihydrofolate reductase-thymidylate synthase, bifunctional TS-DHFR, BmDHFR, dehydrogenase, tetrahydrofolate, DFR-TS, DFR1, DfrA, DfrB, DHFR, DHFR type IIIC, DHFR-TS, DHFR-TS1, DHFR-TS2, DHFR2, DHFRL1, DHFRLS, dihydrofolate reductase, dihydrofolate reductase-like, dihydrofolate reductase-thymidylate synthase, dihydrofolate reductase:thymidylate synthase, dihydrofolic acid reductase, dihydrofolic reductase, EC 1.5.1.4, ecDHFR, folA, folA3, folic acid reductase, folic reductase, FolM, hDHFR, hDHFR-1, hDHFR-2, HjDHFR, hvDHFR1, hvDHFR2, LAU_0427, LBRM_06_0830, mDHFR, mjDHFR, More, myDHFR, NADPH-dihydrofolate reductase, pcDHFR, PKNH_0509600, ppDHFR, pteridine reductase, pteridine reductase:dihydrofolate reductase, PTR2, R-plasmid-encoded dihydrofolate reductase, R67 DHFR, R67 dihydrofolate reductase, reductase, dihydrofolate, S3DHFR, Smdhfr, Smp 175230, spDHFR, svDHFR, tcptr1, tetrahydrofolate dehydrogenase, THY-1, THY-2, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase-dihydrofolate reductase, Trimethoprim resistance protein, TS-DHFR, WUBG_00817

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.3 dihydrofolate reductase

Molecular Weight

Molecular Weight on EC 1.5.1.3 - dihydrofolate reductase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, sucrose density gradient centrifugation
107000
110000
140000
-
gel filtration
147000
gel filtration
14900
-
gel filtration, ultracentrifugation, isoenzyme I
15000
16700
-
PAGE under denaturating and nondenaturating conditions
16900
-
1 * 16900, R-plasmid enzyme, PAGE under denaturating and nondenaturating conditions
17000
-
gel filtration
17300 - 18300
173000
-
1 * 173000, both forms, SDS-PAGE
17643
17900
-
gel filtration
18000
18250
-
MB 1428, amino acid sequence
18290
mass spectrometry
18300
-
amino acid sequence
18500
-
? * 18500, SDS-PAGE, isozyme I and II
18694
-
1 * 18694, electrospray mass spectrometry and calculated, 1 * 20000, SDS-PAGE
18855
Halalkalibacterium halodurans
-
1 * 18855, mass spectrometry, dynamic light scattering
19000
19000 - 21000
-
DNA-sequence dtermination and SDS-PAGE
19600
-
isoenzyme II, amino acid sequence
20000
20000 - 22000
-
human placenta, gel filtration
20157
Halalkalibacterium halodurans
-
x + 18855, wild-type, x * 20157, His-tagged enzyme, mass spectrometry
21000
21300
-
1 * 21300, SDS-PAGE
21450
21460
-
methotrexate resistant L1210(R) cells, amino acid sequence
21500
21650
-
amino acid sequence
22000
22500
23000
23000 - 24000
-
SDS-PAGE
24000
25000
26000
-
sucrose density gradient centrifugation
28000
-
wild type, gel filtration and velocity sedimentation
29000
Tequatrovirus T4
-
SDS-PAGE
35000
-
bacterial isozyme type II, R factor encoded
36000
39000
-
gel filtration
42000
x * 22000, SDS-PAGE of native enzyme. x * 42000, SDS-PAGE of enzyme modified by small ubiquitin-like modifier 1
45000
-
enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function, dihydrofolate reductase subunit, SDS-PAGE
50000
-
2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE
56400
-
2 * 56400, methotrexate-resistant, each subunit is a bifunctional thymidylate synthase-dihydrofolate reductase, SDS-PAGE
56700
-
2 * 56700, bifunctional enzyme: thymidylate synthase-dihydrofolate reductase, SDS-PAGE
57000
x * 57000, recombinant detagged enzyme, SDS-PAGE
58000
-
2 * 58000, isoform IIa, SDS-PAGE
additional information