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1.5.1.20: methylenetetrahydrofolate reductase [NAD(P)H]

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate reductase [NAD(P)H], go to the full flat file.

Word Map on EC 1.5.1.20

Reaction

5-methyltetrahydrofolate
+
NAD(P)+
=
5,10-methylenetetrahydrofolate
 +
NAD(P)H
 +
H+

Synonyms

10-methylenetetrahydrofolate reductase, 5,10-CH2-H4folate reductase, 5,10-methylenetetrahydrofolate reductase, 5,10-methylenetetrahydrofolate reductase (FADH2), 5,10-methylenetetrahydrofolate reductase (NADPH), 5,10-methylenetetrahydrofolic acid reductase, 5,10-methylenetetrahydropteroylglutamate reductase, 5-methylenetetrahydrofolate:NADP+ oxidoreductase, 5-methyltetrahydrofolate:(acceptor) oxidoreductase, 5-methyltetrahydrofolate:NAD oxidoreductase, 5-methyltetrahydrofolate:NAD+ oxidoreductase, 5-methyltetrahydrofolate:NADP+ oxidoreductase, AtMTHFR-1, EC 1.1.1.171, EC 1.1.1.68, EC 1.1.99.15, EC 1.7.99.5, HsMTHFR, MET13, methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide) reductase, methylenetetrahydrofolate reductase, methylenetetrahydrofolate reductase (NADPH), methylenetetrahydrofolic acid reductase, MetVF-type methylenetetrahydrofolate reductase, More, MTHFR, MTHFR2, N5,10-methylenetetrahydrofolate reductase, N5,N10-methylenetetrahydrofolate reductase, NADH:CH2-H4folate oxidoreductase, NapAB, reductase, methylenetetrahydrofolate (reduced nicotinamide adenine dinucleotide phosphate), reductase, methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide), respiratory nitrate reductase, type II-MTHFR

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.20 methylenetetrahydrofolate reductase [NAD(P)H]

Cloned

Cloned on EC 1.5.1.20 - methylenetetrahydrofolate reductase [NAD(P)H]

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning, disruption and expression of the two genes MET12 and MET13 encoding isoenzymes of MTHFR, MET12 is located on chromosome XVI and encodes a 657 amino acids protein, MET13 is located on chromosome VII, encodes a 599 amino acids protein and is responsible for most of the activity in cell, expression in Escherichia coli BL21
-
enzyme is encoded by the 2 genes met9 and met11, which can complement the double-mutant strain, but they cannot complement for each other in the met9-deficient and met11-mutant, respectively, DNA and amino acid sequence determination and analysis
-
expressed in Epstein-Barr virus-transformed lymphoblasts
-
expressed in Escherichia coli and Neuro-2a cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in HepG2 cells
-
expressed in Mus musculus
-
expression of chimeric mutant in an enzyme-deficient strain, overexpression of the plant enzyme from Arabidopsis thaliana in an enzyme-deficient strain can complement the mutant and results in 8fold increased accumulation of S-adenosyl-L-methionine, mechanism overview
expression of recombinant human MTHFR at high levels in Sf9 cells from Spodoptera frugiperda by using a baculovirus expression system
-
expression of the D120N and E28Q mutant plasmids in Escherichia coli strain AB 1909
-
expression of wild-type and mutant enzymes in Escherichia coli strain AB109 and AB1909-(DE3)7D
-
overexpression in strain BL21(DE3)
-
overexpression of the wild-type enzyme and the chimeric mutant in a Saccharomyces cerevisiae enzyme-deficient strain, complementation of the enzyme-deficient strain, 8fold increased accumulation of S-adenosyl-L-methionine in case of recombinant wild-type expression in yeast, mechanism overview
-
two MTHFR genes, a cDNAs encoding functional MTHFR is cloned, sequenced and expressed in Saccharomyces cerevisiae
two MTHFR genes, two cDNAs encoding functional MTHFRs are cloned, sequenced and expressed in Saccharomyces cerevisiae at high levels
-
wild type and several mutants
-