1.5.1.2: pyrroline-5-carboxylate reductase

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For detailed information about pyrroline-5-carboxylate reductase, go to the full flat file.

Reaction

Synonyms

1-pyrroline-5-carboxylate reductase, aldimine reductase, DELTA1-pyrroline-5-carboxylate reductase, DELTA1-pyrroline-5-carboxylate reductase 2, L-proline oxidase, L-proline-NAD(P)+ 5-oxidoreductase, L-proline:NAD(P)+ 5-oxidoreductase, MTR_7g090160, NADPH-L-DELTA'-pyrroline carboxylic acid reductase, P5C reductase, P5CDH, P5CR, PO, POX, PROC, proline oxidase, Pycr1, PYCR2, Pyr5C reductase, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate reductase, reductase, pyrroline-5-carboxylate, TcCLB.509207.90

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.1 With NAD⁺ or NADP⁺ as acceptor

                1.5.1.2 pyrroline-5-carboxylate reductase

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Results	in table
8	Activating Compound
45977	AA Sequence
9	Application
1	CAS Registry Number
23	Cloned(Commentary)
92	Cofactor
8	Crystallization (Commentary)
535	Disease/ Diagnostics
4	Expression
12	General Information
6	General Stability
67	IC50 Value
175	Inhibitors
33	kcat/KM [mM/s]
11	Ki Value [mM]
127	KM Value [mM]
25	Localization
14	Metals/Ions
27	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
116	Organism
9	Pathway
128	PDB ID
41	pH Optimum
7	pH Range
3	pH Stability
1	pI Value
9	Protein Variants
32	Purification (Commentary)
2	Reaction
27	Reaction Type
100	Reference
1	Renatured (Commentary)
82	Source Tissue
42	Specific Activity [micromol/min/mg]
12	Storage Stability
140	Substrates and Products (Substrate)
18	Subunits
70	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
17	Temperature Stability [°C]
52	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.5.1.2 - pyrroline-5-carboxylate reductase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, 50% loss of activity in 4 weeks

Glycine max

-

392125

-15°C, glutathione, EDTA

Bos taurus

-

392132

-18°C, 0.15-0.2 M potassium phosphate buffer pH 7.4, 2 months

Clostridium sporogenes

-

392123

-20°C, 1 mg protein/ml, 50% glycerol, 0.1 mM dithiothreitol, unstable

Saccharomyces cerevisiae

-

392118

-20°C, 50% glycerol, 0.1 mM dithiothreitol, stable for 6 months

Saccharomyces cerevisiae

-

392119

-20°C, inactivation in few days, unstable in the absence of NADPH

Bos taurus

-

392117

-70°C, 50 mM triethanolamine buffer pH 7.4, 9% w/v sucrose, 1 mM EDTA, 2 mM MgCl2, 30 mM 2-mercaptoethanol

Rattus norvegicus

-

392111

-80°C, 1 year

Bos taurus

-

392138

-80°C, 20 mM Caps, pH 9.4, 0.5 M NaCl, 6 months

Homo sapiens

P32322

676969

-80°C, 5% glycerol, 2 mM dithiothreitol, 1 month

Hordeum vulgare

-

392113

3°C, 0.1 M sodium phosphate buffer pH 7.6, 10% (NH4)2SO4, 7 days

Cucurbita moschata

-

392127

4°C, 50% loss of activity in 1 weeks

Glycine max

-

392125